TUMORIGENESIS BY MILLIPORE FILTERS IN MICE - HISTOLOGY AND ULTRASTRUCTURE OF TISSUE REACTIONS AS RELATED TO PORE SIZE
TUMORIGENESIS BY MILLIPORE FILTERS IN MICE - HISTOLOGY AND ULTRASTRUCTURE OF TISSUE REACTIONS AS RELATED TO PORE SIZE
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DOI：
10.1093/jnci/51.4.1275
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发表时间：
1973-01-01
期刊：
影响因子：
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通讯作者：
BRAND, KG
中科院分区：
文献类型：
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作者：
KARP, RD;JOHNSON, KH;BRAND, KG
将直径为2.0 cm、孔径范围为0.025 - 8.0 µm的8种不同微孔的亲水性Millipore滤片皮下植入雌性CBA/H、CBA/H-T6或（CBA/H × CBA/BrA）F1小鼠。在接受孔径≤0.1 µm滤器的31只动物中，有25只在7-27个月后在植入部位出现肉瘤。孔径≥0.22 µm的过滤器无致瘤性。在植入后1、3、5和10个月对由植入物和周围组织反应组成的样本进行组织学和超微结构研究。非致瘤性滤器诱导的组织反应特征为a）巨噬细胞或其细胞质突起侵入滤器，b）巨噬细胞内存在吞噬溶酶体，作为吞噬活性的证据。在致瘤性滤器诱导的组织反应中，这些标准总是缺失;相反，存在更多的纤维化，导致胶原组织囊变厚。结果允许评估各种病因学假设，涉及a）细胞接触或通信中断，和b）异物表面的光滑性，连续性或电荷。
Hydrophilic Millipore filter disks, 2.0 cm in diameter, with 8 different pore sizes ranging from 0.025 to 8.0 µm, were subcutaneously implanted into female CBA/H, CBA/H-T6, or (CBA/H × CBA/BrA)F1mice. Sarcomas at implant sites developed after 7–27 months in 25 of 31 animals that had received filters with pore sizes of ≤0.1 µm. Filters with pore sizes of ≥0.22 µm were non-tumorigenic. Histologic and ultrastructural studies were done 1, 3, 5, and 10 months post implantation on specimens composed of implants and surrounding tissue reactions. Nontumorigenic filters induced tissue reactions characterized bya)invasion of filters by macrophages or their cytoplasmic processes andb)presence of phagolysosomes within macrophages as evidence of phagocytic activity. In tissue reactions induced by tumorigenic filters, these criteria were always missing; instead, there was more fibrosis which resulted in thicker collagenous tissue capsules. The results permitted assessment of various etiologic hypotheses relating toa)interruption of cellular contact or communication, andb)smoothness, continuity, or electric charge of foreign-body surfaces.