Community structure of bacteria associated with sheaths of freshwater and brackish Thioploca species
Community structure of bacteria associated with sheaths of freshwater and brackish Thioploca species
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DOI：
10.1007/s00248-006-9127-8
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发表时间：
2006-11-01
影响因子：
3.6
通讯作者：
Fukui, Manabu
中科院分区：
文献类型：
--
作者：
Kojima, Hisaya;Koizumi, Yoshikazu;Fukui, Manabu
采用变性梯度凝胶电泳（DGGE）技术对两个淡水湖（日本的Biwa湖和德国的Constance湖）和一个半咸水湖泊（日本的Ogawara湖）中Thioploca spp.鞘相关的细菌群落进行了分析。琵琶湖块状沉积物和硫斑菌细丝的DGGE谱带对比表明，存在与硫斑菌鞘相关的特定细菌群落。作为鞘相关群落的成员，从两个淡水湖的样本中检测到属于拟杆菌门的细菌。Biwa湖Thioploca的DGGE条带与Constance湖的DGGE条带关系密切，属于候选分区OP8。与淡水湖的情况相反，小川原湖的硫代菌中未检出拟杆菌门细菌和OP8。而Ogawara湖的2条DGGE谱带与Constance湖的DGGE谱带亲缘关系较近，属于Chloroflexi。从琵瓦湖获得的两条DGGE条带与系统发育上遥远的异化铁（III）还原菌密切相关。对用于DGGE分析的样品进行了亚硫酸盐异化还原酶基因的克隆分析。分析结果表明，淡水/半咸淡水硫藻鞘对大多数硫酸盐还原剂的生态意义不大。
Bacterial communities associated with sheaths of Thioploca spp. from two freshwater lakes (Lake Biwa, Japan, and Lake Constance, Germany) and one brackish lake (Lake Ogawara, Japan) were analyzed with denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene fragments. The comparison between the DGGE band patterns of bulk sediment and Thioploca filaments of Lake Biwa suggested the presence of specific bacterial communities associated with Thioploca sheaths. As members of sheath-associated communities, bacteria belonging to Bacteroidetes were detected from the samples of both freshwater lakes. A DGGE band from Thioploca of Lake Biwa, belonging to candidate division OP8, was quite closely related to another DGGE band detected from that of Lake Constance. In contrast to the case of freshwater lakes, no bacterium of Bacteroidetes or OP8 was detected from Thioploca of Lake Ogawara. However, two DGGE bands from Lake Ogawara, belonging to Chloroflexi, were quite closely related to a DGGE band from Lake Constance. Two DGGE bands obtained from Lake Biwa were closely related to phylogenetically distant dissimilatory Fe(III)-reducing bacteria. Cloning analyses for a dissimilatory sulfite reductase gene were performed on the same samples used for DGGE analysis. The results of the analyses suggest that sheaths of freshwater/brackish Thioploca have little ecological significance for the majority of sulfate reducers.