FoxO is required for optimal fitness of the migratory brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)
FoxO is required for optimal fitness of the migratory brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)
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DOI：
10.1111/1744-7917.13163
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发表时间：
2022-12
期刊：
影响因子：
4
通讯作者：
Jin-Li Zhang;Ke‐Liang Liu;Xin-Yu Cai;Xin-Yang Liu;Hai-Jun Xu
中科院分区：
文献类型：
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作者：
Jin-Li Zhang;Ke‐Liang Liu;Xin-Yu Cai;Xin-Yang Liu;Hai-Jun Xu
叉头盒 O (FoxO) 蛋白是胰岛素/胰岛素样信号通路下游的主要转录效应子，调节许多发育和生理过程。 FoxO 中具有功能丧失突变的全变态昆虫表现出与使用 RNA 干扰的半变态昆虫不同的表型。尽管 FoxO 具有重要的功能，但半变态昆虫是否与全变态昆虫共享 FoxO 的进化保守功能仍有待澄清。我们使用成簇规则间隔的短回文重复序列/CRISPR相关9（CRISPR/Cas9）基因组编辑系统，建立了翅二态性褐飞虱（BPH）Nilaparvata lugens的纯合FoxO缺失突变体（NlFoxO4E），褐飞虱是一种重要的稻田经济害虫。 NlFoxO4E突变体的表型包括若虫持续时间延长、寿命缩短、繁殖减少和抗逆性降低。此外，NlFoxO 的消耗促进了翅芽中的细胞增殖，并导致 100% 长翅变体，这与短翅野生型 BPH 形成鲜明对比。这些发现表明，NlFoxO 与其在全变态昆虫中的对应物在功能上高度保守，并且是褐飞虱最佳适应性所必需的。 FoxO 研究的见解可能有助于识别 BPH 控制应用的潜在靶基因。
The forkhead box O (FoxO) protein is the main transcriptional effector downstream of the insulin/insulin‐like signaling pathway and regulates many developmental and physiological processes. Holometabolous insects with loss‐of‐function mutations in FoxO exhibit phenotypes distinct from those of hemimetabolous insects in which RNA interference was used. Despite the functional importance of FoxO, whether hemimetabolous insects share an evolutionally conserved function of FoxO with holometabolous insects remains to be clarified. We used the clustered regularly interspaced short palindromic repeats/CRISPR‐associated 9 (CRISPR/Cas9) genome editing‐system to establish a homozygous FoxO‐null mutant (NlFoxO4E) of the wing‐dimorphic brown planthopper (BPH) Nilaparvata lugens, an economically important insect pest of rice fields. The phenotypes of NlFoxO4E mutants included extended nymphal duration, shortened lifespan, reduced reproduction, and decreased stress resistance. In addition, depletion of NlFoxO promoted cell proliferation in wing buds and led to 100% long‐winged morphs, in stark contrast to short‐winged wild‐type BPHs. These findings indicate that NlFoxO is highly functionally conserved with its counterpart in holometabolous insects, and is required for optimal fitness of N. lugens. The insights from FoxO studies may facilitate the identification of potential target genes for BPH control applications.