Bacterial biosensors for evaluating potential impacts of estrogenic endocrine disrupting compounds in multiple species.
Bacterial biosensors for evaluating potential impacts of estrogenic endocrine disrupting compounds in multiple species.
复制标题
用于评估雌激素内分泌干扰化合物对多个物种的潜在影响的细菌生物传感器。
DOI：
10.1002/tox.20708
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发表时间：
2013
影响因子：
4.5
通讯作者：
Wood,DavidW
中科院分区：
文献类型：
--
作者：
Gierach,Izabela;Shapero,Kayle;Eyster,ThomasW;Wood,DavidW
为了研究可疑内分泌干扰化合物(EDCs)的影响和可能的机制，人们开发了各种检测方法。在这项工作中，我们从Solea solea(SOLE)和Sus scrofa(PIG)获得了整合β亚型雌激素受体(ERβ)配体结合域(LBD)的工程大肠杆菌生物传感器菌株。这些菌株通过生长表型的变化来指示这些受体的配体的存在，并可以区分激动剂和拮抗剂，并通过剂量-反应曲线给出结合亲和力的大致指示。将所得菌株与我们之前报道的人类智者β生物传感器菌株进行了比较。在初步测试中，这三株菌株都正确地鉴定出具有高度确定性(Z‘通常大于0.5)的雌激素试验化合物，包括弱结合试验化合物双酚A(Z’≈0.1-0.3)。该菌株中传感元件的模块化设计允许通过简单的LBD交换来快速开发新的基于物种的生物传感器，这表明它用于跨多个物种的EDC影响的初步比较分析。有趣的是，生物传感器菌株的生长表型表明，对高度雌激素样的对照化合物具有类似的结合，但对于结合较弱的内皮细胞，则在配体结合方面存在差异。2011Wiley期刊，Inc.环境毒物，2013年。
To study the effects and possible mechanisms of suspected endocrine disrupting compounds (EDCs), a wide variety of assays have been developed. In this work, we generated engineeredEscherichia colibiosensor strains that incorporate the ligand‐binding domains (LBDs) of the β‐subtype estrogen receptors (ERβ) fromSolea solea(sole), andSus scrofa(pig). These strains indicate the presence of ligands for these receptors by changes in growth phenotype, and can differentiate agonist from antagonist and give a rough indication of binding affinity via dose‐response curves. The resulting strains were compared with our previously reportedHomo sapiensERβ biosensor strain. In initial tests, all three of the strains correctly identified estrogenic test compounds with a high degree of certainly (Z′ typically greater than 0.5), including the weakly binding test compound bisphenol A (BPA) (Z′ ≈ 0.1–0.3). The modular design of the sensing element in this strain allows quick development of new species‐based biosensors by simple LBD swapping, suggesting its use in initial comparative analysis of EDC impacts across multiple species. Interestingly, the growth phenotypes of the biosensor strains indicate similar binding for highly estrogenic control compounds, but suggest differences in ligand binding for more weakly binding EDCs. © 2011 Wiley Periodicals, Inc. Environ Toxicol, 2013.