Comparison of pancreas juice proteins from cancer versus pancreatitis using quantitative proteomic analysis
Comparison of pancreas juice proteins from cancer versus pancreatitis using quantitative proteomic analysis
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DOI：
10.1097/01.mpa.0000240615.20474.fd
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发表时间：
2007-01-01
期刊：
影响因子：
2.9
通讯作者：
Brentnall, Teresa A.
中科院分区：
文献类型：
--
作者：
Chen, Ru;Pan, Sheng;Brentnall, Teresa A.
目的：胰腺炎是胰腺的一种炎症性疾病。然而，它通常与胰腺癌共享许多分子特征。胰腺癌中存在的生物标志物经常发生在胰腺炎的情况下。因此，开发胰腺癌诊断生物标志物的努力已经复杂的假阳性参与pancreat.Methods：在试图开发胰腺癌的蛋白质生物标志物，我们以前使用定量蛋白质组学，以确定和量化的蛋白质胰腺癌汁。胰液是胰腺导管细胞分泌蛋白质的丰富来源。在这项研究中，我们使用了类似的方法来识别和定量蛋白质从胰腺炎juice.Results：在总数，72蛋白质被确定和定量胰腺炎患者与合并正常对照果汁的胰腺汁的比较。19个汁液蛋白质过表达，8个在胰腺炎汁液中低表达，与正常胰液相比至少低表达2倍。在这27个胰腺炎差异表达蛋白中，有9个蛋白在胰腺癌患者的胰液中也有差异表达。结论：从胰腺炎患者的胰液中鉴定这些差异表达蛋白，为进一步研究胰腺炎相关蛋白和排除胰腺癌的假阳性生物标志物提供了有用的信息。
Objectives: Pancreatitis is an inflammatory condition of the pancreas. However, it often shares many molecular features with pancreatic cancer. Biomarkers present in pancreatic cancer frequently occur in the setting of pancreatitis. The efforts to develop diagnostic biomarkers for pancreatic cancer have thus been complicated by the false-positive involvement of pancreatitis.Methods: In an attempt to develop protein biomarkers for pancreatic cancer, we previously use quantitative proteomics to identify and quantify the proteins from pancreatic cancer juice. Pancreatic juice is a rich source of proteins that are shed by the pancreatic ductal cells. In this study, we used a similar approach to identify and quantify proteins from pancreatitis juice.Results: In total, 72 proteins were identified and quantified in the comparison of pancreatic juice from pancreatitis patients versus pooled normal control juice. Nineteen of the juice proteins were overexpressed, and 8 were underexpressed in pancreatitis juice by at least 2-fold compared with normal pancreatic juice. Of these 27 differentially expressed proteins in pancreatitis, 9 proteins were also differentially expressed in the pancreatic juice from pancreatic cancer patient.Conclusions: Identification of these differentially expressed proteins from pancreatitis juice provides useful information for future study of specific pancreatitis-associated proteins and to eliminate potential false-positive biomarkers for pancreatic cancer.