Glial and neuronal connexin expression patterns in the rat spinal cord during development and following injury
Glial and neuronal connexin expression patterns in the rat spinal cord during development and following injury
复制标题
DOI：
10.1002/cne.20567
复制
发表时间：
2005-08-15
影响因子：
2.5
通讯作者：
Olson, L
中科院分区：
文献类型：
--
作者：
Lee, IH;Lindqvist, E;Olson, L
随着时间的推移，脊髓损伤会诱发一系列复杂的退行性和重塑事件。脊髓损伤（SCI）后通过间隙连接改变细胞间通讯的可能作用仍然相对未被探索。采用原位杂交和免疫组化的方法研究了大鼠新生脊髓、成年正常脊髓和成年损伤脊髓中间隙连接基因和蛋白连接蛋白43 （Cx43）、Cx36和Cx32的时空表达模式。Cx36在未成熟神经元中强烈表达，在发育过程中水平显著下降，而Cx43和Cx32在成年期持续表达。在成人脊髓完全横断后，Cx43 mRNA和蛋白水平在数小时内上调，特别是在损伤后4周的灰质吻侧，达到正常水平的3倍以上。Cx43的免疫反应性主要见于星形胶质细胞，很少见于小胶质细胞。相比之下，Cx36和Cx32 mRNA和蛋白在脊髓损伤后沿整个脊髓轴相对稀疏且不变。Cx43是成人中枢神经系统中最丰富的间隙连接蛋白，已被证明在星形胶质细胞之间以及星形胶质细胞与少突胶质细胞之间形成通道。反应性星形胶质细胞中Cx43的长期上调可能是脊髓损伤后局部星形胶质网络重排的一个重要组成部分。(c) 2005 Wiley-Liss, Inc。
Spinal cord injury induces a complex cascade of degenerative and remodeling events evolving over time. The possible roles of changed intercellular communication via gap junctions after spinal cord injury (SCI) have remained relatively unexplored. We investigated the temporospatial expression patterns of gap junctional genes and proteins, connexin 43 (Cx43), Cx36, and Cx32, by in situ hybridization and immunohistochemistry in the rat neonatal, adult normal, and adult injured spinal cord. Cx36 was strongly expressed in immature neurons, and levels declined markedly during development, whereas Cx43 and Cx32 persisted throughout adulthood. After a complete transection of the adult spinal cord, the levels of Cx43 mRNA and protein were up-regulated within hours, especially in gray matter rostral to the lesion, reaching over three times normal levels at 4 weeks postinjury. Cx43 immunoreactivity was seen primarily in astrocytes and rarely in microglia. In contrast, Cx36 and Cx32 mRNA and proteins were relatively sparse and unchanged after spinal cord injury along the entire axis of the spinal cord. Cx43 is the most abundant gap junctional protein in the adult CNS and has been shown to form channels between astrocytes as well as between astrocytes and oligodendrocytes. Long-term up-regulation of Cx43 in reactive astrocytes may be one critical component in the rearrangement of the local astroglial network following SCI. (c) 2005 Wiley-Liss, Inc.