Bronchoalveolar Lavage Fluid-Derived Exosomes: A Novel Role Contributing to Lung Cancer Growth
Bronchoalveolar Lavage Fluid-Derived Exosomes: A Novel Role Contributing to Lung Cancer Growth
复制标题
支气管肺泡灌洗液衍生的外泌体：促进肺癌生长的新作用
DOI：
10.3389/fonc.2019.00197
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发表时间：
2019-04
影响因子：
4.7
通讯作者：
Dong Li
中科院分区：
文献类型：
--
作者：
Yibao Yang;Ping Ji;Xuan Wang;Hao Zhou;Junlu Wu;Wenqing Quan;Anquan Shang;Junjun Sun;Chenzheng Gu;Jenni Firrman;Weidong Xiao;Zujun Sun;Dong Li
外泌体是由许多不同细胞类型产生的纳米囊泡，被视为细胞间通讯的重要介质。尽管支气管肺泡灌洗液（BALF）已被证明与肿瘤的发生有关，但其在肺癌（LC）中的作用仍不清楚。在本文中，我们系统地研究了 LC 中 BALF 衍生的外泌体。 C57BL/6 小鼠被注射 Lewis 肺癌细胞并暴露于不可分型流感嗜血杆菌 (NTHi) 裂解物。分析表明，与对照组（仅暴露在空气中）相比，这些小鼠肺部肿瘤的生长显着增强。来自小鼠 BALF 的外泌体的表征表明，暴露于 NTHi 裂解物的小鼠中肿瘤坏死因子 α 和白细胞介素 6 的水平升高。此外，异常的 BALF 衍生的外泌体促进了 LC 的体外和体内发育。 BALF 衍生的外泌体的内化有助于 LC 肿瘤的发展。总的来说，我们的数据表明 BALF 中的外泌体是参与肺癌生长和进展的关键因素。
Exosomes are nanovesicles produced by a number of different cell types and regarded as important mediators of cell-to-cell communication. Although bronchoalveolar lavage fluid (BALF) has been shown to be involved in the development of tumors, its role in lung cancer (LC) remains unclear. In this article, we systemically studied BALF-derived exosomes in LC. C57BL/6 mice were injected with Lewis lung carcinoma cells and exposed to non-typeable Haemophilus influenza (NTHi) lysate. The analysis showed that the growth of lung tumors in these mice was significantly enhanced compared with the control cohort (only exposure to air). Characterization of the exosomes derived from mouse BALF demonstrated elevated levels of tumor necrosis factor alpha and interleukin-6 in mice exposed to NTHi lysates. Furthermore, abnormal BALF-derived exosomes facilitated the development of LC in vitro and in vivo. The internalization of the BALF-derived exosomes contributed to the development of LC tumors. Collectively, our data demonstrated that exosomes in BALF are a key factor involved in the growth and progression of lung cancer.
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DOI：
10.1073/pnas.1303906110
发表时间：
2013-07-30
影响因子：
11.1
作者：
Meckes, David G., Jr.;Gunawardena, Harsha P.;Raab-Traub, Nancy
通讯作者：
Raab-Traub, Nancy
影响因子：
11.5
作者：
Qin, Wenyi;Tsukasaki, Yoshikazu;Sauter, Edward R.
通讯作者：
Sauter, Edward R.
影响因子：
4.4
作者：
Prado, Noela;Marazuela, Eva G.;Batanero, Eva
通讯作者：
Batanero, Eva
影响因子：
12.4
作者：
Paredes, P. Torregrosa;Esser, J.;Gabrielsson, S.
通讯作者：
Gabrielsson, S.
影响因子：
8
作者：
Li, D.;Beisswenger, C.;Bals, R.
通讯作者：
Bals, R.