Nucleoli From Growing Oocytes Inhibit the Maturation of Enucleolated, Full-Grown Oocytes in the Pig
Nucleoli From Growing Oocytes Inhibit the Maturation of Enucleolated, Full-Grown Oocytes in the Pig
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DOI：
10.1002/mrd.21320
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发表时间：
2011-06-01
影响因子：
2.5
通讯作者：
Fulka, Josef, Jr.
中科院分区：
文献类型：
--
作者：
Kyogoku, Hirohisa;Ogushi, Sugako;Fulka, Josef, Jr.
在哺乳动物中，成熟卵母细胞的核仁是胚胎发育所必需的，但不是卵母细胞成熟所必需的。在我们的研究中，通过去除核仁和/或将核仁转移到先前去核、生长(直径约100 mm)或完全发育(120 Mm)的猪卵母细胞中，研究了生长中的卵母细胞核仁在卵母细胞成熟中的作用。在第一个实验中，从核仁被放线菌素D处理致密的生长中的卵母细胞中提取核仁，并在体外成熟去核的卵母细胞。大多数未处理或经放线菌素D处理的卵母细胞未发生生发泡破裂(GVBD分别为13%和12%)。然而，去核、生长中的卵母细胞的GVBD率显著增加到46%。再次注射来自成熟卵母细胞的核仁(23%)可以恢复去核发育卵母细胞的低GVBD发生率(23%)，但将来自完全发育的卵母细胞的核仁重新注入去核生长卵母细胞(49%)则不能恢复。去核后的成熟卵母细胞注入成熟卵母细胞或成熟卵母细胞的核仁后，生发泡中的核仁发生重组(分别为73%和60%)。成熟后，注射成熟卵母细胞核仁的去核完全成熟卵母细胞成熟到中期II期(56%)，而注射生长卵母细胞核仁使这一成熟度降低到21%。这些结果表明，生长中的卵母细胞核仁参与了卵母细胞减数分裂的停滞，而完全发育的卵母细胞核仁已经失去了这一能力。摩尔。是Reprod。戴夫。2011年，78：426-435。(C)2011年Wiley-Liss，Inc.
In mammals, the nucleolus of full-grown oocyte is essential for embryonic development but not for oocyte maturation. In our study, the role of the growing oocyte nucleolus in oocyte maturation was examined by nucleolus removal and/or transfer into previously enucleolated, growing (around 100 mm in diameter) or full-grown (120 mm) pig oocytes. In the first experiment, the nucleoli were aspirated from growing oocytes whose nucleoli had been compacted by actinomycin D treatment, and the enucleolated oocytes were matured in vitro. Most of non-treated or actinomycin D-treated oocytes did not undergo germinal vesicle breakdown (GVBD; 13% and 12%, respectively). However, the GVBD rate of enucleolated, growing oocytes significantly increased to 46%. The low GVBD rate of enucleolated, growing oocytes was restored again by the re-injection of nucleoli from growing oocytes (23%), but not when nucleoli from full-grown oocytes were re-injected into enucleolated, growing oocytes (49%). When enucleolated, full-grown oocytes were injected with nucleoli from growing or full-grown oocytes, the nucleolus in the germinal vesicle was reassembled (73% and 60%, respectively). After maturation, the enucleolated, full-grown oocytes injected with nucleoli from full-grown oocytes matured to metaphase II (56%), whereas injection with growing-oocyte nucleoli reduced this maturation to 21%. These results suggest that the growing-oocyte nucleolus is involved in the oocyte's meiotic arrest, and that the full-grown oocyte nucleolus has lost the ability. Mol. Reprod. Dev. 78: 426-435, 2011. (C) 2011 Wiley-Liss, Inc.