TOPOGRAPHY OF BINDING-SITES FOR NEUROHYPOPHYSEAL HORMONES IN RAT-BRAIN
TOPOGRAPHY OF BINDING-SITES FOR NEUROHYPOPHYSEAL HORMONES IN RAT-BRAIN
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DOI：
10.1016/0014-2999(85)90036-6
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发表时间：
1985-01-01
影响因子：
5
通讯作者：
TERLOU, M
中科院分区：
文献类型：
--
作者：
DEKLOET, ER;ROTTEVEEL, F;TERLOU, M
用氚标记的配体与32 μm脑切片体外孵育，用LKB Ultrofilm放射自显影，在大鼠脑神经解剖学定义的区域内检测到精氨酸加压素（AVP）和催产素（OT）的结合位点。通过胶片图像的计算机化密度测定法对位点进行定量。在海马腹侧下托、杏仁中央核、外侧隔、嗅核和孤束核中[~ 3 H] AVP标记结合位点最多，而齿状回中含有大量低亲和力结合位点。[3 H]OT的结合模式较为离散。OT结合位点在杏仁中央核、腹侧下托和嗅核中的浓度相对较高。外侧隔和孤束核几乎没有位移OT结合，而没有显着的关联OT与齿状回被发现。地形的神经垂体激素的结合位点对应于本地化的响应网站在行为，生化和电生理研究。
Binding sites for arginine-vasopressin (AVP) and oxytocin (OT) were detected in neuroanatomically defined regions of rat brain by using in vitro incubation of 32 μm brain sections with the tritium-labelled ligands and autoradiography with LKB Ultrofilm. Sites were quantified by computerized densitometry of the film images. The highest amount of [3H]AVP-labelled binding sites was observed in the ventral subiculum of the hippocampus, central amygdala nucleus, lateral septum, olfactory nucleus and nucleus tractus solitarii, while the dentate gyrus contained a large amount of low affinity binding. The binding pattern of [3H]OT was more discrete. Relatively high concentrations of OT binding sites were observed in the central amygdala, ventral subiculum and olfactory nucleus. The lateral septum and the nucleus tractus solitarii were virtually devoid of displaceable OT binding while no significant association of OT with the dentate gyrus was found. The topography of binding sites for neurohypophyseal hormones corresponds to the localization of responsive sites in behavioural, biochemical and electrophysiological studies.