Vanadate inhibits Feo-mediated iron transport in Vibrio cholerae.
Vanadate inhibits Feo-mediated iron transport in Vibrio cholerae.
复制标题
钒酸盐抑制霍乱弧菌中 Feo 介导的铁转运。
DOI：
10.1093/mtomcs/mfab059
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Payne,ShelleyM
中科院分区：
文献类型：
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作者：
Shin,Minhye;Gomez-Garzon,Camilo;Payne,ShelleyM
铁是霍乱弧菌生存的必需元素，而Feo作为亚铁运输的主要细菌系统，对该病原菌在低氧环境中的生长具有重要意义。为了深入了解其生化机制，我们评估了广泛使用的ATP酶抑制剂对V的N-末端结构域的ATP水解活性的影响。霍乱结果表明，钒酸钠和叠氮化钠能有效抑制V的N端结构域的催化活性。霍乱钒酸钠是更有效的抑制剂。体内胆铁转运这些结果有助于更全面地了解FeO功能的生物化学，并为设计针对细菌FeoB蛋白的有效抑制剂提供了依据。
Iron is an essential element forVibrio choleraeto survive, and Feo, the major bacterial system for ferrous iron transport, is important for growth of this pathogen in low-oxygen environments. To gain insight into its biochemical mechanism, we evaluated the effects of widely used ATPase inhibitors on the ATP hydrolysis activity of the N-terminal domain ofV. choleraeFeoB. Our results showed that sodium orthovanadate and sodium azide effectively inhibit the catalytic activity of the N-terminal domain ofV. choleraeFeoB. Further, sodium orthovanadate was the more effective inhibitor againstV. choleraeferrous iron transportin vivo. These results contribute to a more comprehensive biochemical understanding of Feo function, and shed light on designing effective inhibitors against bacterial FeoB proteins.