Hydrocarbon and Lipid Microbiology Protocols - Petroleum, Hydrocarbon and Lipid Analysis
Hydrocarbon and Lipid Microbiology Protocols - Petroleum, Hydrocarbon and Lipid Analysis
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碳氢化合物和脂质微生物学方案 - 石油、碳氢化合物和脂质分析
DOI：
10.1007/8623_2016_206
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Franchini F
中科院分区：
文献类型：
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作者：
Franchini F
水生环境会产生一系列挥发性有机化合物（VOCs），这些化合物可以转移到大气中并影响气候。我们对海水挥发物生物地球化学的认识主要来源于生物源微量气体二甲基硫化物（DMS）的研究。在这里，我们描述了四种用于定量水样中DMS和其他VOCs的方案，这些方案在分离和定量之前使用气相色谱火焰光度检测（GC-FPD）使用直接注射或低温富集技术。只需进行少量调整，就可以对方案进行定制，以量化一系列其他气体，包括异戊二烯和乙烯等碳氢化合物，或氯甲烷或溴仿等卤代烃。DMS的定量限为1.5 pmol，灵敏度范围为0.2 ~ 20 μM（顶空直接进样200 μL）， 50 ~ 250 nM（顶空吹扫1.92 mL气相），0.5 ~ 350 nM（瓶内吹扫3ml水相），和亚纳摩尔范围内的管内净化样品体积高达200毫升。另外两个方案的调整包括定量生物dms前体二甲基磺酰丙酸（DMSP）和dms氧化产物二甲基亚砜（DMSO）。
Aquatic environments produce a range of volatile organic compounds (VOCs) that can transfer into the atmosphere and affect climate. Much of our understanding on the biogeochemistry of volatiles in seawater stems from research on the biogenic trace gas dimethyl sulfide (DMS). Here, we describe four protocols for the quantification of DMS and other VOCs in aqueous samples that utilise direct injection or cryogenic enrichment techniques before separation and quantification using gas chromatography with flame photometric detection (GC-FPD). With few adjustments, the protocols can be customised to quantify a range of other gases including hydrocarbons such as isoprene and ethene, or halocarbons such as methyl chloride or bromoform. The limit of quantification for DMS is 1.5 pmol and the protocols range in sensitivities for DMS from 0.2 to 20 μM (direct injection of 200 μL headspace), 50 to 250 nM (headspace purging of 1.92 mL gaseous phase), 0.5 to 350 nM (in-vialpurging of 3 mL aqueous phase), and the sub-nanomolar range forin-tubepurging of sample volumes up to 200 mL. Two additional adaptations of the protocol include quantification of the biological DMS-precursor dimethylsulfoniopropionate (DMSP) and the DMS-oxidation product dimethyl sulfoxide (DMSO).