Differential localization of septins in the mouse brain
Differential localization of septins in the mouse brain
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DOI：
10.1002/1096-9861(20001211)428:2
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发表时间：
2000-12
影响因子：
2.5
通讯作者：
A. Kinoshita;M. Noda;M. Kinoshita
中科院分区：
文献类型：
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作者：
A. Kinoshita;M. Noda;M. Kinoshita
我们对septin家族的四个成员CDCrel‐1、septin 6、CDC10和H5进行了比较免疫组织化学研究，这四个成员在成年小鼠大脑中大量表达。我们发现，在光镜和电镜水平上，每个septin都表现出重叠但不同的分布。CDCrel‐1在抑制性突触前终末丰富，并与丘脑、苍白球和小脑核中的gaba能囊泡有关。Septin6与包括嗅球肾小球在内的多个脑区突触囊泡有关。CDC10在大脑中弥漫性表达，并定位于突触前膜和星形胶质突下。H5定位于大脑某些特定区域的星形胶质突起。这些隔素的差异表达和亚细胞定位表明，给定的神经元或神经胶质细胞表达一组特定的隔素单体，并且由此产生的具有不同成分的隔素复合物可能在大脑中发挥不同的作用。[j] .中华神经科杂志，2000,28(4):323 - 323。©2000 Wiley‐Liss, Inc。
We have carried out a comparative immunohistochemical study on four members of the septin family, CDCrel‐1, Septin6, CDC10, and H5, which are abundantly expressed in the adult mouse brain. We found that each septin showed overlapping but distinct distribution at the levels of light and electron microscopy. CDCrel‐1 was abundant in inhibitory presynaptic terminals and associated with GABAergic vesicles in the thalamus, globus pallidus, and cerebellar nuclei. Septin6 was associated with synaptic vesicles in various brain regions, including glomeruli of the olfactory bulb. CDC10 was diffusely expressed in the brain and was localized beneath presynaptic membrane and astroglial processes. H5 was localized in the astroglial processes in some specific brain regions. The differential expression and subcellular localization of these septins indicates that a given neuron or glial cell expresses a specific set of septin monomers and that the resulting septin complexes with distinct compositions may play distinct roles in the brain. J. Comp. Neurol. 428:223–239, 2000. © 2000 Wiley‐Liss, Inc.