Structure and in situ organisation of the Pyrococcus furiosus archaellum machinery
Structure and in situ organisation of the Pyrococcus furiosus archaellum machinery
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DOI：
10.7554/elife.27470
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发表时间：
2017-06-27
期刊：
影响因子：
7.7
通讯作者：
Kuhlbrandt, Werner
中科院分区：
文献类型：
--
作者：
Daum, Bertram;Vonck, Janet;Kuhlbrandt, Werner
古菌是古菌用于推进或表面粘附的大分子机器，使它们能够增殖和入侵新的领土。古菌的分子组成和驱动它的马达似乎与功能相当的细菌鞭毛和鞭毛马达完全不同。然而，古生动物机械的结构却鲜为人知。利用电子冷冻显微镜（cryoEM）的组合模式，我们在4.2埃分辨率下解决了火焦球菌古菌细丝的结构，并在原位可视化了其运动复合物的结构和组织。这使我们能够建立一个结合古菌及其运动复合体的结构模型，为从分子角度理解古菌的游泳运动铺平了道路。
The archaellum is the macromolecular machinery that Archaea use for propulsion or surface adhesion, enabling them to proliferate and invade new territories. The molecular composition of the archaellum and of the motor that drives it appears to be entirely distinct from that of the functionally equivalent bacterial flagellum and flagellar motor. Yet, the structure of the archaellum machinery is scarcely known. Using combined modes of electron cryo-microscopy (cryoEM), we have solved the structure of the Pyrococcus furiosus archaellum filament at 4.2 angstrom resolution and visualise the architecture and organisation of its motor complex in situ. This allows us to build a structural model combining the archaellum and its motor complex, paving the way to a molecular understanding of archaeal swimming motion.