Nuclear organization and dynamics of DNA replication in eukaryotes
Nuclear organization and dynamics of DNA replication in eukaryotes
复制标题
DOI：
10.2741/3600
复制
发表时间：
2009-06-01
影响因子：
3.1
通讯作者：
Zink, Daniele
中科院分区：
文献类型：
--
作者：
Muck, Joscha;Zink, Daniele
复制焦点是细胞核中 DNA 复制的单位。它们拥有复制机制和活跃的复制子。单个复制子的缠绕似乎发生在芽殖酵母的复制焦点处。哺乳动物细胞核中与分叉进展相关的动力学在很大程度上尚不清楚，但大规模的 DNA 缠绕并没有发生。复制焦点被排列成时空高阶模式。在不同的真核类群中观察到了不同种类的此类模式，但在哺乳动物中观察到的特征模式是高度保守的。在哺乳动物中，S期进展过程中复制灶高阶模式的变化是由于复制机器与相邻稳定定位的染色质结构域的顺序关联，这导致复制灶在相邻核位点的顺序形成。这些发现表明复制焦点的时空模式是由潜在的基因组结构决定的。未来的研究必须解决基因组结构到底以何种方式参与 DNA 复制的空间和时间调控的问题。
Replication foci are the units of DNA replication in the nucleus. They harbor the replication machinery and activereplicons. Spooling of individual replicons appears to occur at replication foci of budding yeast. The dynamics associated with fork progression in mammalian nuclei are largely unclear but large-scale spooling of DNA does not occur. Replication foci are arranged into spatio-temporal higher-order patterns. Different kinds of such patterns have been observed in different eukaryotic taxa but the characteristic patterns observed in mammals are highly conserved. The changes of higher-order patterns of replication foci during S phase progression are in mammals due to the sequential association of the replication machinery with neighboring stably positioned chromatin domains, which leads to the sequential formation of replication foci at neighboring nuclear sites. These findings suggest that the spatio-temporal patterns of replication foci are determined by the underlying genome architecture. Future studies will have to address the question in which way exactly genome architecture is involved in the spatial and temporal regulation of DNA replication.