DIFFERENTIAL DISTRIBUTION OF CILIATED EPITHELIAL-CELLS IN TRACHEA OF HAMSTERS - IMPLICATIONS FOR STUDIES OF PATHOGENESIS
DIFFERENTIAL DISTRIBUTION OF CILIATED EPITHELIAL-CELLS IN TRACHEA OF HAMSTERS - IMPLICATIONS FOR STUDIES OF PATHOGENESIS
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DOI：
10.1093/infdis/135.1.9
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发表时间：
1977-01-01
影响因子：
6.4
通讯作者：
CAMERON, AM
中科院分区：
文献类型：
--
作者：
GABRIDGE, MG;AGEE, CC;CAMERON, AM
通过扫描电子显微镜检查成年仓鼠气管内部的形态。在上皮层中观察到相对较大的无纤毛细胞斑块。这些斑块覆盖了数十万平方微米，在气管腹侧表面最为明显，尤其是中间三分之一处。仓鼠中这些无纤毛细胞区域（无论是孤立的还是成片的）的频率比小鼠、大鼠或猫要高得多。在软骨环开口端之间的气管肌条上观察到仓鼠气管中最大的纤毛。根据四唑还原测定的测量，纤毛最重的区域也具有最大的细胞代谢活性。 3H标记的肺炎支原体细胞的附着与广泛的纤毛呈负相关，因为在气管表面的中间三分之一和腹侧区域发现了最大数量的计数。仓鼠呼吸道上皮纤毛的区域差异可能会影响肺炎支原体的发病机制和分离研究，因此在实验设计中应考虑和控制这些差异。
The morphology of the inner aspect of the adult hamster trachea was examined by scanning electron microscopy. Relatively large patches of unciliated cells were observed in the epithelial layer. The patches, which covered several hundreds of thousands of square microns, were most conspicuous on the ventral surface of the trachea, especially in the middle third. The frequency of these areas of unciliated cells, both isolated and in patches, was much greater in hamsters than in mice, rats or cats. Greatest ciliation in the hamster trachea was observed over the strip of trachealis muscle between the open ends of the cartilaginous rings. Areas with the heaviest ciliation also had the greatest activity of cellular metabolism, as measured by the tetrazolium reduction assay. The attachment of 3H-labeled cells of Mycoplasma pneumoniae was inversely correlated with extensive ciliation, since the greatest numbers of counts were found on the middle third and ventral regions of the tracheal surface. The regional differences in ciliation of respiratory epithelium in hamsters may influence studies of pathogenesis and isolation of M. pneumoniae and these differences should therefore be considered and controlled in the experimental design.