Genomic organization of a novel glycosylphosphatidylinositol MAM gene expressed in human tissues and tumors
Genomic organization of a novel glycosylphosphatidylinositol MAM gene expressed in human tissues and tumors
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DOI：
10.1038/sj.onc.1205383
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发表时间：
2002-05-02
期刊：
影响因子：
8
通讯作者：
Benito, M
中科院分区：
文献类型：
--
作者：
De Juan, C;Iniesta, P;Benito, M
我们报告了一个新的人类基因的基因组组织映射到染色体6p21，编码一个假定的糖基磷脂酰肌醇（GPI）锚定蛋白含有MAM（meprin，A5抗原，蛋白酪氨酸磷酸酶mu）域，我们已被称为GPIM（GPI和MAM）蛋白。GPIM基因全长8.9kb，由17个外显子组成，全长约65.5kb。推导的多肽由955个氨基酸组成，并表现出在不同类型的细胞粘附分子（CAM）中发现的结构特征，如免疫球蛋白结构域的存在，MAM结构域的存在或通过GPI基序锚到细胞膜的能力。在正常人体组织中的表达分析表明，该基因表达为5kb和9.5kb的mRNA。此外，较小的转录物在一些人类癌细胞系以及不同的原发性肿瘤（肺、结肠、子宫、胃和乳腺）中高度表达。有趣的是，该基因在分析的几种肿瘤组织中的表达高于其相应的正常组织。因此，GPIM是一个新的基因，编码一种具有某些CAM结构特征的蛋白质，可能参与肿瘤的进展。
We report the genomic organization of a novel human gene mapped to chromosome 6p21, encoding a putative glycosylphosphatidylinositol (GPI) anchored protein containing a MAM (meprin, A5 antigen, protein tyrosine phosphatase mu) domain, that we have termed as GPIM (GPI and MAM) protein. GPIM gene consists of an 8.9 kb transcript composed of 17 coding exons spanning about 65.5 kb of genomic DNA. The deduced polypeptide consists of 955 amino acids and exhibits structural features found in different types of cell adhesion molecules (CAMs), such as the presence of immunoglobulin domains, the presence of a MAM domain or the capacity to anchor to the cell membrane by a GPI motif. Expression analysis in normal human tissues revealed that this gene is expressed as a 5 kb and 9.5 kb mRNA. Furthermore, the smaller transcript is highly expressed in some human cancer cell lines, as well as in different primary tumors (lung, colon, uterus, stomach and breast). Interestingly, the gene was higher expressed in several tumor tissues analysed as compared to their corresponding normal tissues. Thus, GPIM is a novel gene codifying a protein with structural features characteristics of some CAMs, which might be involved in the tumor progression.