Thermoresponsive self-assembly of nanostructures from a collagen-like peptide-containing diblock copolymer.
Thermoresponsive self-assembly of nanostructures from a collagen-like peptide-containing diblock copolymer.
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DOI：
10.1002/mabi.201400358
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发表时间：
2015-01
影响因子：
4.6
通讯作者：
Kiick KL
中科院分区：
文献类型：
--
作者：
Luo T;He L;Theato P;Kiick KL
温度触发形成具有不同生物活性的纳米结构提供了选择性修饰基质和药物递送的机会。为了这些目的，产生包含与三螺旋形成胶原蛋白样肽（CLP）缀合的聚（二甘醇甲基醚甲基丙烯酸酯）（PDEGMEMA）的二嵌段聚合物。CLP结构域在与聚合物缀合后保持其三螺旋构象的能力通过圆二色性（CD）确认。动态光散射（DLS）测量表明二嵌段缀合物在水溶液中经历可逆的温度诱导转变以形成直径约为IOOnm的纳米颗粒，转变温度为37 °C。透射电子显微镜（TEM）表明，在转变温度以上形成定义明确的囊泡，而在室温下没有观察到超分子组装。PDEGMEMA-CLP二嵌段的自组装是由温度响应结构域在其LCST以上的塌陷触发的。CLP结构域在这些纳米结构中的掺入可能为含胶原基质的选择性靶向提供机会。
Temperature-triggered formation of nanostructures with distinct biological activity offers opportunities in selective modification of matrices and in drug delivery. Toward these ends, diblock polymers comprising poly(diethylene glycol methyl ether methacrylate) (PDEGMEMA) conjugated to a triple helix-forming collagen-like peptide (CLP) is produced. The ability of the CLP domain to maintain its triple helix conformation after conjugation with the polymer is confirmed via circular dichroism (CD). Dynamic light scattering (DLS) measurements suggest the diblock conjugate undergo a reversible temperature-induced transition in aqueous solution to form nanoparticles with a diameter of approximately 100 nm, with a transition temperature of 37 °C. Transmission electron microscopy (TEM) suggests the formation of well-defined vesicles above the transition temperature, while no supramolecular assemblies are observed at room temperature. The self-assembly of PDEGMEMA-CLP diblock is triggered by the collapse of the thermoresponsive domain above its LCST. The incorporation of CLP domains in these nanostructures may offer opportunities for the selective targeting of collagen-containing matrices.