Image Correlation-Based Method to Assess Ciliary Beat Frequency in Human Airway Organoids
Image Correlation-Based Method to Assess Ciliary Beat Frequency in Human Airway Organoids
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DOI：
10.1109/tmi.2021.3112992
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发表时间：
2022-02-01
影响因子：
10.6
通讯作者：
Kim, Jun Ki
中科院分区：
文献类型：
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作者：
Choi, Woo June;Yoon, Jung-Ki;Kim, Jun Ki
人气道内的纤毛运动对于维持清洁的肺环境是必不可少的。运动纤毛具有特征性的纤毛搏动频率（CBF）。然而，CBF测量与当前的视频显微镜技术可以是容易出错的，由于使用的单点傅立叶变换，这往往是有偏见的纤毛测量。在这里，我们描述了一种新的视频显微镜技术，利用CBF分析的运动对比度成像和图像相关性的度量。它可以提供客观和选择性的CBF测量个别运动纤毛，并生成CBF地图的成像区域。我们的方法的测量性能进行了验证，在体外人体气道类器官模型，模拟一个实际的人体气道上皮细胞。所确定的感兴趣区域（ROI）的CBF等于手动计数获得的CBF。没有观察到常规方法的信号冗余问题。此外，所获得的CBF测量是稳健的光学焦移，并表现出空间异质性和温度依赖性。该技术可用于评估呼吸道中的纤毛运动，并确定患者的纤毛运动是非同步的还是非周期性的。因此，我们的观察表明，所提出的方法可以在临床上适用于作为一种筛选工具，以诊断纤毛病变。
Ciliary movements within the human airway are essential for maintaining a clean lung environment. Motile cilia have a characteristic ciliary beat frequency (CBF). However, CBF measurement with current video microscopic techniques can be error-prone due to the use of the single-point Fourier transformation, which is often biased for ciliary measurements. Herein, we describe a new video microscopy technique that harnesses a metric of motion-contrast imaging and image correlation for CBF analysis. It can provide objective and selective CBF measurements for individual motile cilia and generate CBF maps for the imaged area. The measurement performance of our methodology was validated with in vitro human airway organoid models that simulated an actual human airway epithelium. The CBF determined for the region of interest (ROI) was equal to that obtained with manual counting. The signal redundancy problem of conventional methods was not observed. Moreover, the obtained CBF measurements were robust to optical focal shifts, and exhibited spatial heterogeneity and temperature dependence. This technique can be used to evaluate ciliary movement in respiratory tracts and determine whether it is non-synchronous or aperiodic in patients. Therefore, our observations suggest that the proposed method can be clinically adapted as a screening tool to diagnose ciliopathies.