Effects of metals on enantioselective toxicity and biotransformation of cis-bifenthrin in zebrafish
Effects of metals on enantioselective toxicity and biotransformation of cis-bifenthrin in zebrafish
复制标题
金属对顺式联苯菊酯在斑马鱼体内的对映选择性毒性和生物转化的影响
DOI：
10.1002/etc.3747
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发表时间：
2017
影响因子：
4.1
通讯作者：
Liu Jing
中科院分区：
文献类型：
--
作者：
Yang Ye;Ji Dapeng;Huang Xin;Zhang Jianyun;Liu Jing
据报道，拟除虫菊酯和金属在流域中的共存对水生物种构成了巨大风险。拟除虫菊酯是一类手性杀虫剂，具有对映体选择性毒性和生物转化性。然而，金属对拟除虫菊酯的对映选择性的影响尚未得到评价。研究了环境相关浓度下镉（Cd）、铜（Cu）和铅（Pb）对顺式联苯菊酯（cis-BF）在斑马鱼体内对映体选择性毒性和代谢的影响。添加Cd、Cu或Pb显著增加了cis-BF处理组的外消旋体和R-对映体中斑马鱼的死亡率。在外消旋-顺式-BF-或1 R-顺式-BF-处理组中，添加Cd、Cu或Pb导致斑马鱼中对映体分数（EF）减少，R-对映体残留比例增加。在1 S-cis-BF处理组中，同时暴露于Cd导致EF降低和S-对映体残留水平降低。此外，共暴露于3种金属导致各对映体生物降解特性不同，细胞色素P450（CYP）1、CYP 2和CYP 3基因表达发生差异，这可能是cis-BF在斑马鱼体内对映体选择性生物降解的原因。这些结果表明，在水生环境中手性拟除虫菊酯的生态风险评估中应考虑共存金属的影响。Environ Toxicol Chem 2017;36：2139-2146。联系我们_SETAC
Co‐occurrence of pyrethroids and metals in watersheds previously has been reported to pose great risk to aquatic species. Pyrethroids are a class of chiral insecticides that have been shown to have enantioselective toxicity and biotransformation. However, the influence of metals on enantioselectivity of pyrethroids has not yet been evaluated. In the present study, the effects of cadmium (Cd), copper (Cu), and lead (Pb) on the enantioselective toxicity and metabolism ofcis‐bifenthrin (cis‐BF) were investigated in zebrafish at environmentally relevant concentrations. The addition of Cd, Cu, or Pb significantly increased the mortality of zebrafish in racemate andR‐enantiomer ofcis‐BF–treated groups. Inrac‐cis‐BF– or 1R‐cis‐BF–treated groups, the addition of Cd, Cu, or Pb caused a decrease in enantiomeric fraction (EF) and an increased ratio ofR‐enantiomer residues in zebrafish. In 1S‐cis‐BF–treated groups, coexposure to Cd led to a lower EF and decreased residue levels ofS‐enantiomer. In addition, coexposure to the 3 metals resulted in different biodegradation characteristics of each enantiomer accompanied with differential changes in the expression of cytochrome P450 (CYP)1, CYP2, and CYP3 genes, which might be responsible for the enantioselective biodegradation ofcis‐BF in zebrafish. These results suggest that the influence of coexistent metals should be considered in the ecological risk assessment of chiral pyrethroids in aquatic environments.Environ Toxicol Chem2017;36:2139–2146. © 2017 SETAC