Prevalence of Cryptosporidium parvum infection and pattern of oocyst shedding in calves in Japan
Prevalence of Cryptosporidium parvum infection and pattern of oocyst shedding in calves in Japan
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DOI：
10.1016/s0304-4017(00)00338-1
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发表时间：
2000-12-20
影响因子：
2.6
通讯作者：
Ono, K
中科院分区：
文献类型：
--
作者：
Uga, S;Matsuo, J;Ono, K
本试验对犊牛隐孢子虫感染和卵囊排出规律进行了观察。少于或等于30天）。蔗糖离心漂浮技术显示28/30（93%）的小牛通过了冷孢子虫卵囊。在出生后第六天首次检测到卵囊脱落，8%的小牛检测呈阳性。这一比率逐日增加，到第15天达到约80%。卵囊脱落时间为1 ~ 13 d，平均7 d。感染C.感染小隐孢子虫的犊牛腹泻率（33%）显著高于未感染犊牛（8%）（P< 0.05）。可能是细小病毒感染30日龄小牛排泄的卵囊平均数量约为每克粪便6 × 10（7）个。这些结果表明，一头小牛在出生后的第一个月内将排出约6 × 10（11）个卵囊，考虑到每天的粪便量和排泄时间。因此，很明显，小牛在隐孢子虫病向小牛和人类的传播中是重要的。(C)2000 Elsevier Science B. V.保留所有权利。
Cryptosporidium parvum infection and the pattern of oocyst shedding were observed in calves, A total of 480 fecal samples were collected from 30 calves (age. less than or equal to 30 days) over a period of 10 months from June 1998 to March 1999. A sucrose centrifugal flotation technique revealed 28/30 (93%) calves were passing Cryprosporidium oocysts. Oocyst shedding was first detected on the sixth day after birth, with 8% of the calves testing positive. This rate increased day by day and reached approximately 80% by day 15. Oocyst shedding varied from 1 to 13 days, with a mean of 7 days. Calves infected with C. parvum had a significantly higher rate of diarrhea (33%) than non-infected calves (8%) (P< 0.05), suggesting C. parvum infection as the likely cause. The mean number of oocysts excreted by calves 30 days old was approximately 6 x 10(7) per gram of feces. These results indicated that one calf would excrete some 6 x 10(11) oocysts in the first month after birth, taking both the quantity of feces in a day and the period of excretion into consideration. Accordingly, it is clear that calves are important in the spread of cryptosporidiosis to calves and humans. (C) 2000 Elsevier Science B.V. All rights reserved.