Two Novel Exopolysaccharides from Bacillus amyloliquefaciens C-1: Antioxidation and Effect on Oxidative Stress
Two Novel Exopolysaccharides from Bacillus amyloliquefaciens C-1: Antioxidation and Effect on Oxidative Stress
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DOI：
10.1007/s00284-014-0717-2
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发表时间：
2014-11
影响因子：
2.6
通讯作者：
Haixia Yang;Jianjun Deng;Yue Yuan;Daidi Fan;Y. Zhang;Ruijuan Zhang;B. Han
中科院分区：
文献类型：
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作者：
Haixia Yang;Jianjun Deng;Yue Yuan;Daidi Fan;Y. Zhang;Ruijuan Zhang;B. Han
从一株新分离的解淀粉芽孢杆菌C-1菌株中分离得到两个无臭水溶性胞外多糖(EPS)组分，分别为EPS-1和EPS-2。纯化的EPS-1含有葡萄糖/甘露糖/半乳糖/阿拉伯糖的相对比例为15：4：2：1，相对分子质量为79.6 kDa；而EPS-2仅含有葡萄糖和甘露糖，其相对分子质量为19.8 kDa。抗氧化活性结果表明，多糖1具有较强的还原能力、清除超氧阴离子自由基(O2−·)和清除羟基自由基(OH·)的活性。对于H_2O_2诱导的HepG2细胞损伤，EPS-1显著减少细胞内活性氧的生成，降低细胞内丙二醛水平，恢复细胞内超氧化物歧化酶活性。对于EPS-2，没有检测到抗氧化剂活性。所有这些结果综合表明，只有C-1产生的EPS-1作为一种天然抗氧化剂，才有相当大的潜力用于医用化合物或功能添加剂。
Two odorless, water-soluble exopolysaccharide (EPS) fractions, EPS-1 and EPS-2, were isolated from a newly isolatedBacillus amyloliquefaciensstrain C-1 and purified by ion exchange and gel chromatography. The purified EPS-1 contained glucose/mannose/galactose/arabinose in a relative proportion of 15:4:2:1, and possessed a molecular weight of 79.6 kDa, while EPS-2 contained only glucose and mannose in a 3:1 ratio, with the molecular weights of 19.8 kDa. The antioxidant activity results showed that EPS-1 exhibited strong reducing power, superoxide radicals (O2−·), and hydroxyl free radicals (OH·) scavenging activities. For the H2O2-induced injury in HepG2 cells, EPS-1 significantly decreased the formation of reactive oxygen species, intracellular malondialdehyde levels, and restored intracellular superoxide dismutase activity. For EPS-2, there had no detectable antioxidant activities. And all these results collectively showed that as a natural antioxidant, only EPS-1 produced by C-1 had considerable potential to be used as medical compounds or functional additives.