Patient-derived tumor immune microenvironments in patient-derived xenografts of lung cancer.
Patient-derived tumor immune microenvironments in patient-derived xenografts of lung cancer.
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DOI：
10.1186/s12967-018-1704-3
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发表时间：
2018-11-26
影响因子：
7.4
通讯作者：
Fang B
中科院分区：
文献类型：
--
作者：
Pu X;Zhang R;Wang L;Chen Y;Xu Y;Pataer A;Meraz IM;Zhang X;Wu S;Wu L;Su D;Mao W;Heymach JV;Roth JA;Swisher SG;Fang B
Because patient-derived xenografts (PDXs) are grown in immunodeficient mouse strains, PDXs are regarded as lacking an immune microenvironment. However, whether patients’ immune cells co-exist in PDXs remains uncharacterized. We cultured small pieces of lung PDX tissue in media containing human interleukin-2 and characterized the proliferated lymphocytes by flow cytometric assays with antibodies specific for human immune cell surface markers. Presence of immune cells in PDXs was also determined by immunohistochemical staining. Human tumor-infiltrating lymphocytes (TILs) were cultured from nine of 25 PDX samples (36%). The mean time of PDX growth in immunodeficient mice before obtaining TILs in culture was 113 days (range 63–292 days). The TILs detected in PDXs were predominantly human CD8+ T cells, CD4+ T cells, or CD19+ B cells, depending on cases. DNA fingerprint analysis showed that the TILs originated from the same patients as the PDXs. Further analysis of two PDX-derived CD8+ T cells showed that they were PD-1−, CD45RO+, and either CD62L+ or CD62L−, suggesting they were likely memory T cells. Immunohistochemical staining showed that human T cells (CD8+ or CD4+), B cells (CD19+), and macrophages (CD68+) were present in stroma or intraepithelial cancer structures and that human PD-L1 was expressed in stromal cells. Moreover, the patient-derived immune cells in PDX can be passaged to the F2 generation and may migrate to spleens of PDX-bearing mice. Patient-derived immune cells co-exist in early passages of PDXs in some lung cancer PDX models. The CD8+ cells from PDXs were likely memory T cells. These results suggest that PDXs can be used for evaluating the functionality of immune components in tumor microenvironments. The online version of this article (10.1186/s12967-018-1704-3) contains supplementary material, which is available to authorized users.
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影响因子：
82.9
作者：
Gao, Hui;Korn, Joshua M.;Sellers, William R.
通讯作者：
Sellers, William R.
DOI：
10.1016/s1470-2045(16)30053-5
发表时间：
2016-07
期刊：
The Lancet. Oncology
影响因子：
--
作者：
Goldberg SB;Gettinger SN;Mahajan A;Chiang AC;Herbst RS;Sznol M;Tsiouris AJ;Cohen J;Vortmeyer A;Jilaveanu L;Yu J;Hegde U;Speaker S;Madura M;Ralabate A;Rivera A;Rowen E;Gerrish H;Yao X;Chiang V;Kluger HM
通讯作者：
Kluger HM
影响因子：
28.2
作者：
Girotti, Maria Romina;Gremel, Gabriela;Marais, Richard
通讯作者：
Marais, Richard
影响因子：
3.7
作者：
Radaelli E;Hermans E;Omodho L;Francis A;Vander Borght S;Marine JC;van den Oord J;Amant F
通讯作者：
Amant F
DOI：
10.1097/cji.0b013e3181fad2b0
发表时间：
2010-11
期刊：
Journal of immunotherapy (Hagerstown, Md. : 1997)
影响因子：
--
作者：
Inozume T;Hanada K;Wang QJ;Ahmadzadeh M;Wunderlich JR;Rosenberg SA;Yang JC
通讯作者：
Yang JC