Exosome detection via the ultrafast-isolation system: EXODUS
Exosome detection via the ultrafast-isolation system: EXODUS
复制标题
DOI：
10.1038/s41592-020-01034-x
复制
发表时间：
2021-01-11
期刊：
影响因子：
48
通讯作者：
Liu, Fei
中科院分区：
文献类型：
--
作者：
Chen, Yuchao;Zhu, Qingfu;Liu, Fei
外泌体在疾病诊断和治疗中显示出巨大的潜力。然而，目前的分离方法是繁琐的，并且受到低速度、产率和纯度的影响，限制了基础研究和临床应用。在这里，我们描述了一种有效的外泌体检测方法，通过超快速分离系统（EXODUS），允许自动化的无标记纯化外泌体从不同的生物流体。我们通过负压振荡和双耦合谐波振荡器使能的膜振动获得了外泌体的超高效纯化。我们的两个耦合振荡器在膜上产生双频横波，使EXODUS在速度、纯度和产量方面优于其他隔离技术。我们通过从113名患者的尿液样本中纯化外泌体来证明EXODUS，并通过高分辨率能力和高通量分析验证了外泌体RNA谱分析的实际相关性。
Exosomes have shown great potential in disease diagnostics and therapeutics. However, current isolation approaches are burdensome and suffer from low speed, yield and purity, limiting basic research and clinical applications. Here, we describe an efficient exosome detection method via the ultrafast-isolation system (EXODUS) that allows automated label-free purification of exosomes from varied biofluids. We obtained the ultra-efficient purification of exosomes by negative pressure oscillation and double coupled harmonic oscillator-enabled membrane vibration. Our two coupled oscillators generate dual-frequency transverse waves on the membranes, enabling EXODUS to outperform other isolation techniques in speed, purity and yield. We demonstrated EXODUS by purifying exosomes from urine samples of 113 patients and validated the practical relevance in exosomal RNA profiling with the high-resolution capability and high-throughput analysis.