The conditional inhibition of gene expression in cultured Drosophila cells by antisense RNA.
The conditional inhibition of gene expression in cultured Drosophila cells by antisense RNA.
复制标题
反义RNA对培养的果蝇细胞中基因表达的条件抑制。
DOI：
10.1093/nar/17.23.9761
复制
发表时间：
1989
影响因子：
14.9
通讯作者：
Goldstein,LS
中科院分区：
文献类型：
--
作者：
Bunch,TA;Goldstein,LS
Genes producing antisense RNA are becoming important tools for the selective inhibition of gene expression. Experiments in different biological systems, targeting different mRNAs have yielded diverse results with respect to the success of the technique and its mechanism of action. We have examined the potential of three antisense genes, whose transcription is driven by aDrosophilametallothionein promoter, to inhibit the expression of alcohol dehydrogenase (ADH) or a microtubule associated protein (205K MAP) in culturedDrosophilacells. Expression of ADH was significantly reduced upon induction of the anti-ADH genes. The ADH mRNA does not appear to be destabilized by the presence of antisense RNA but rather exists at similar levels in hybrid form. Hybrids are detected with both spliced and unspliced ADH RNA. In contrast to these results, antisense genes producing antisense RNA in great excess to 205K MAP mRNA, which is itself far less abundant than the ADH mRNA, failed to show any inhibition of 205K MAP expression.
