Fluorometric determination of serum and urinary aluminium with 8-quinolinol by kinetic-differentiation-mode micellar chromatography.
Fluorometric determination of serum and urinary aluminium with 8-quinolinol by kinetic-differentiation-mode micellar chromatography.
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通过动力学分化模式胶束色谱法用 8-羟基喹啉荧光测定血清和尿铝。
DOI：
10.1016/s0021-9673(97)00510-4
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
T. Yotsuyanagi
中科院分区：
文献类型：
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作者：
M. Sato;H. Yoshimura;T. Shimmura;H. Obi;S. Hatakeyama;E. Kaneko;H. Hoshino;T. Yotsuyanagi
建立了8-喹啉-8-喹啉胶束层析测定铝的新方法。该方法无需预先去蛋白，即可测定人血清和尿液中的铝，最低可达1μg/L。该方法最显著的特点是在金属离子中只有铝离子发生选择性反应。在室温下，血清中铝与8-喹啉酚的络合物在几分钟内即可形成。采用反相柱，以乙腈、十二烷基硫酸钠和Triton X-100为洗脱剂，在Ex 370 nm，Em 504 nm波长处检测。该方法与塞曼石墨炉原子吸收光谱法测定结果一致。该方法为医学领域中铝的测定提供了一种简便、快速的方法。
A new fluorometric method has been developed for the determination of aluminium with 8-quinolinol by kinetic-differentiation-mode micellar chromatography. The proposed method enabled the determination of aluminium down to 1 μg/l in human serum and urine without preliminary deproteinization. The most remarkable point of this method is that only aluminium ion selectively responds among metal ions. The complex formation of aluminium in serum with 8-quinolinol was completed within a few minutes at room temperature. The serum matrix and aluminium chelate were separated on a reversed-phase column with an eluent containing acetonitrile, sodium dodecylsulfate, and Triton X-100, and aluminium chelate was detected at Ex 370 nm, Em 504 nm. The values obtained by this method were in good agreement with those of Zeeman graphite-furnace atomic absorption spectrometry. The proposed method will provide a simple and rapid technique for the determination aluminium in medical fields.
Hitoshi Hoshino：“基于三乙醇胺存在下 4-(2-吡啶偶氮基)间苯二酚成色的离子对反相高效液相色谱法选择性测定痕量铬 (III)”。
DOI：
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发表时间：
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期刊：
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