Rapid identification of dysregulated genes in cutaneous malignant melanoma metastases using cDNA technology
Rapid identification of dysregulated genes in cutaneous malignant melanoma metastases using cDNA technology
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DOI：
10.1159/000079985
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发表时间：
2004-01-01
影响因子：
2.7
通讯作者：
Hengge, UR
中科院分区：
文献类型：
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作者：
Mirmohammadsadegh, A;Baer, A;Hengge, UR
DNA微阵列技术的一项重要应用是同时分析不同mRNA的基因表达。比较患病组织和健康组织的 mRNA 模式可能有助于了解特定疾病的发病机制。在癌症组织中，鉴定出的失调基因可以作为诊断或预后的新分子标记，或者理想地可以作为治疗的新靶点。使用膜 cDNA 阵列技术，我们分析了人类黑色素瘤的基因表达，黑色素瘤是最具侵袭性的癌症类型之一，具有高转移潜力，并且在全球范围内发病率显着增加。为了解释肿瘤的异质性，我们将 10 名不同患者的皮肤黑色素瘤转移灶的总 RNA 与原代人类黑色素细胞进行了比较。大量基因失调（上调/下调），例如涉及凋亡基因生长因子受体结合蛋白 10、Bcl2 相关 X 膜蛋白、细胞死亡的 Bcl2 拮抗剂、谷胱甘肽 S 转移酶 theta(1) 和谷胱甘肽还原酶。最终，黑色素瘤相关基因的鉴定可能为识别和靶向恶性黑色素瘤提供潜在的治疗策略。版权所有 (C) 2004 S. Karger AG，巴塞尔。
One important application of DNA microarray technology is the simultaneous analysis of gene expression of different mRNAs. Comparison of mRNA patterns of diseased and healthy tissue may help to understand the pathogenesis of a given disorder. In cancer tissue, identified dysregulated genes may serve as new molecular markers for diagnosis or prognosis or may ideally serve as new targets for therapy. Using membrane cDNA array technology, we analyzed gene expression in human melanomas, one of the most aggressive types of cancer with a high metastatic potential and with markedly increased incidence worldwide. To account for the heterogeneity of tumors, we compared total RNA from cutaneous melanoma metastases of 10 different patients with primary human melanocytes. An abundance of genes was dysregulated (up-/downregulated), which involved for example the apoptosis gene growth factor receptor-bound protein 10, Bcl2-associated X membrane protein, Bcl2 antagonist of cell death, glutathione S-transferase theta(1) and glutathione reductase. Ultimately, the identification of melanoma-associated genes may provide a potential therapeutic strategy for identifying and targeting malignant melanoma. Copyright (C) 2004 S. Karger AG, Basel.