The diffusional transport of water and small solutes in isolated endothelial cells and erythrocytes.
The diffusional transport of water and small solutes in isolated endothelial cells and erythrocytes.
复制标题
水和小溶质在分离的内皮细胞和红细胞中的扩散运输。
DOI：
10.1016/0005-2736(93)90166-w
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Chinard,FP
中科院分区：
文献类型：
--
作者：
Garrick,RA;Ryan,US;Bower,V;Cua,WO;Chinard,FP
在11.5、15、20和37℃的温度下，用线性扩散技术测量了分离的小牛肺动脉内皮细胞和狗红细胞中氚化水（3 HHO） 14 c -安替比林（AP）和14 c -碘安替比林（IAP）的扩散渗透系数P D。在每种温度下，两种细胞群的P D值依次为3 HHO> IAP> AP。与内皮细胞相比，红细胞中水的P - D更高。红细胞和内皮细胞中AP和IAP的P - D差异并不显著，类似于比较相同溶质通过不同组成的双层的渗透所见的差异。内皮细胞计算的P - D值与分离的毛细血管和全肺结构内皮的P - D值的比较验证了分离细胞作为原位内皮细胞模型的使用。用顺式异戊酸或胆固醇孵育内皮细胞可使水和安替比林的P - D减少。这些数据是根据斯托克扩散和非斯托克扩散进行分析的。最符合这些数据的解释是，膜的磷脂区，而不是碳氢化合物核心，对这些溶质的渗透提供了最大的阻力。
The diffusional permeability coefficients, P D, for tritiated water (3 HHO) 14 C-antipyrine (AP) and 14 C-iodoantipyrine (IAP) in isolated calf pulmonary artery endothelial cells and dog erythrocytes are measured with the linear diffusion technique at 11.5, 15, 20 and 37° C. The P D values for both cell populations follow the sequence 3 HHO> IAP> AP at each of the temperatures. P D for water is higher in the erythrocyte compared to the endothelial cells. The differences in P D for AP and IAP in the erythrocytes and endothelial cells are not dramatic and are similar to the differences seen in comparing permeation of the same solute through bilayers of different composition. A comparison of the values of P D calculated for the endothelial cells with those for isolated capillaries and the structured endothelium in whole lungs validates the use of the isolated cells as models for the endothelial cells in situ. Incubation of the endothelial cells with cis-vaccenic acid or cholesterol produces a reduction in P D for water and antipyrine. These data are analyzed in terms of Stokesian and non-Stokesian diffusion. The interpretation which best accommodates the data is that the phospholipid area of the membrane, rather than the hydrocarbon core, provides the greatest resistance to permeation for these solutes.