THE DISTRIBUTION OF VIMENTIN AND KERATIN IN EPITHELIAL AND NONEPITHELIAL NEOPLASMS - A COMPREHENSIVE IMMUNOHISTOCHEMICAL STUDY ON FORMALIN-FIXED AND ALCOHOL-FIXED TUMORS
THE DISTRIBUTION OF VIMENTIN AND KERATIN IN EPITHELIAL AND NONEPITHELIAL NEOPLASMS - A COMPREHENSIVE IMMUNOHISTOCHEMICAL STUDY ON FORMALIN-FIXED AND ALCOHOL-FIXED TUMORS
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DOI：
10.1093/ajcp/88.3.286
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发表时间：
1987-09-01
影响因子：
3.5
通讯作者：
BATTIFORA, H
中科院分区：
文献类型：
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作者：
AZUMI, N;BATTIFORA, H
波形蛋白被认为是正常间充质细胞和肿瘤间充质细胞的中间纤维，角蛋白被认为是上皮细胞和由其衍生的肿瘤的典型特征。然而，组织培养或渗出液中的许多上皮细胞，以及一些实体上皮肿瘤如肾、子宫内膜、卵巢、肺和甲状腺腺癌中的细胞，已被证明共表达波形蛋白和角蛋白。最近的单克隆抗体，在石蜡包埋组织中的工作相当不错的可用性，使我们进行了一个全面的免疫组织化学研究福尔马林和酒精固定标本的肿瘤中，我们使用的波形蛋白的单克隆抗体。这些结果与我们以前的研究，其中相同的肿瘤组织进行了研究，使用抗角蛋白抗体进行了比较。波形蛋白的抗原性被发现保存在所有酒精固定的标本和63%的福尔马林固定的组织。波形蛋白是几乎所有肉瘤、脑膜瘤、神经鞘瘤和黑色素瘤中唯一的中间丝。此外，不同百分比（10-57%）的癌、神经内分泌瘤、神经母细胞瘤、胸腺瘤和间皮瘤波形蛋白阳性，除神经母细胞瘤外，波形蛋白与角蛋白共表达。在腺癌中，超过50%的甲状腺乳头状癌以及肾癌、子宫内膜癌、卵巢癌和肺癌共表达角蛋白和波形蛋白。一个独特的核旁和基底定位的波形蛋白观察到在许多这些肿瘤的细胞，在主要的角蛋白的顶端分布。作者得出结论，波形蛋白和角蛋白的共表达比以前报道的更广泛，波形蛋白抗体本身对上皮和间叶肿瘤的区分价值有限。
Vimentin has been regarded as the intermediate filament characteristic of normal and neoplastic mesenchymal cells and keratins as typical of epithelial cells and the neoplasms derived from them. However, many epithelial cells in tissue culture or in effusion, as well as cells in some solid epithelial neoplasms such as renal, endometrial; ovarian, pulmonary, and thyroid adenocarcinomas, have been shown to coexpress vimentin and keratin. The recent availability of monoclonal antibodies that work reasonably well in paraffin-embedded tissue led us to carry out a comprehensive immunohistochemical study on formalin- and alcohol-fixed specimens of neoplasms in which we used monoclonal antibodies against vimentin. These results were compared with our previously study in which the same tumor tissues were investigated using antibodies against keratin. The antigenicity of vimentin was found to be preserved in all alcohol-fixed specimens and in 63% of formalin-fixed tissues. Vimentin was the sole intermediate filament present in virtually all sarcomas, meningiomas, schwannomas, and melanomas. In addition, variable percentages (10-57%) of carcinomas, neuroendocrine, neuroblastomas, thymomas, and mesotheliomas were positive for vimentin, which, excpet in the neuroblastomas, was coexpressed with keratins. Among the adenocarcinomas, more than 50% of papillary carcinomas of the thyroid, as well as renal, endometrial, ovarian, and lung carcinomas, coexpressed keratins and vimentin. A distinctive paranuclear and basal localization of vimentin was observed in the cells of many of these tumors, in contrast to the predominantly apical distribution of the keratins. The authors conclude that coexpression of vimentin and keratin is more widespread then previously reported and that antibodies to vimentin, by themselves, are of limited value for the differentiation of epithelial from mesenchymal neoplasms.