The role of nickel accumulation and epithelial cell proliferation in orthodontic treatment-induced gingival overgrowth
The role of nickel accumulation and epithelial cell proliferation in orthodontic treatment-induced gingival overgrowth
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DOI：
10.1093/ejo/cjm074
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发表时间：
2007-12-01
影响因子：
2.6
通讯作者：
Sayal, Ahmet
中科院分区：
文献类型：
--
作者：
Gursoy, Ulvi Kahraman;Sokucu, Oral;Sayal, Ahmet
这项研究的目的是调查镍在正畸治疗引起的牙龈增生中的作用。分析有无过度生长的牙龈组织中镍浓度、过度生长的组织病理学和上皮细胞对不同镍浓度的增殖反应。10名接受正畸治疗的患者(8名女性和2名男性，平均年龄15.4岁)包括在研究中。来自同一患者的增生性和健康牙龈样本。采用原子吸收光谱分析技术测定牙周组织样品中镍的含量。同时对牙龈切除过程中切除的增生区组织进行组织学分析。为分析镍对角质形成细胞增殖的影响，将0.5、2、5和10mUg的镍与角质形成细胞共同孵育11d。统计分析采用Mann-Whitney U检验、方差分析和Tukey‘s检验。结果显示，研究组和对照组牙龈组织中镍浓度没有任何差异，但组织学分析显示，低剂量镍浓度使牙龈上皮厚度增加，上皮细胞增殖显著增加(P=0.031.0 5，0.0 2，0.0 2)，而高剂量镍浓度有毒性反应。在这项研究的局限性中，持续的低剂量镍向上皮细胞释放的影响可能是正畸治疗引起牙龈过度生长的始动因素。
The aim of this study was to investigate the role of nickel in orthodontic treatment-induced gingival hyperplasia. The nickel concentration in gingival tissues with and without overgrowth, histopathology of gingival overgrowths, and epithelial cell proliferation response to different nickel concentrations were analysed. Ten patients receiving orthodontic therapy (eight females and two males, mean age 15.4 years) were included in the study. Hyperplastic and healthy gingiva samples were collected from the same patients. The amount of nickel in the gingival tissue samples was analysed using the atomic absorption spectrometry technique. The tissues removed from hyperplastic areas during gingivectomy were also used for histological analysis. To analyse the effect of nickel on epithelial cell proliferation, four different nickel concentrations (0.5, 2, 5, and 10 mu g) were incubated with keratinocyte cells for 11 days. Mann-Whitney U-test, analysis of variance, and Tukey's test were used in the statistical analyses.The results did not show any difference in nickel concentration between the study and control gingiva tissue samples, but histological analysis demonstrated an increase in epithelial thickness and a significant increase (P = 0.031, 0.02, 0.02) in epithelial cell proliferation in response to low-dose nickel concentrations, with a toxic response to a higher dose. In the limitations of this study, it is plausible that the effect of a continuing low-dose nickel release to epithelium is the initiating factor of gingival overgrowth induced by orthodontic treatment.