Identification of vacuolar phosphate influx transporters in Brassica napus
Identification of vacuolar phosphate influx transporters in Brassica napus
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DOI：
10.1111/pce.14423
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发表时间：
2022-09-01
影响因子：
7.3
通讯作者：
Shi, Lei
中科院分区：
文献类型：
--
作者：
Han, Bei;Wang, Chuang;Shi, Lei
近年来的研究表明，液泡膜磷转运蛋白（vacuolar Pi transporters，VPTs）在拟南芥和水稻细胞磷稳态中起着重要作用，但对VPTs在油菜细胞磷稳态中的作用还不清楚。在这里，我们确定了两个液泡Pi内流转运蛋白B。napus、BnA 09 PHT 5;1b和BnCnPHT 5;1b的功能分析揭示了它们在细胞内Pi稳态中的必要性。这两种芸苔属蛋白质都是拟南芥AtPHT 5;1的同源物，具有相似的序列、结构、液泡膜定位和VPT活性。与野生型B相比，芸苔属pht 5; 1 B双突变体具有较小的芽和较大的芽细胞Pi浓度。这与先前对拟南芥pht 5;1突变体的研究形成对比，表明PHT 5;1-VPTs在B幼苗的细胞Pi稳态中发挥不同的作用。油菜和A. thaliana. BnPHT 5的破坏; 1b基因还引起花器官中的Pi毒性，降低种子产量并影响种子性状，这与AtPHT 5;1在拟南芥花Pi体内稳态中的作用一致。综上所述，我们的研究确定了两个液泡Pi内流转运蛋白在B。揭示了BnPHT 5;1bs在这一植物物种细胞磷稳态中独特而保守的作用。
Recent progress has shown that vacuolar Pi transporters (VPTs) are important for cellular Pi homoeostasis in Arabidopsis thaliana and Oryza sativa under fluctuating external Pi supply, but the identity and involvement of VPTs in cellular Pi homoeostasis in Brassica napus is poorly understood. Here, we identified two vacuolar Pi influx transporters B. napus, BnA09PHT5;1b and BnCnPHT5;1b, and uncovered their necessity for cellular Pi homoeostasis through functional analysis. Both Brassica proteins are homologs of Arabidopsis AtPHT5;1 with a similar sequence, structure, tonoplast localization, and VPT activity. Brassica pht5;1b double mutants had smaller shoots and larger shoot cellular Pi concentrations than wild-type B. napus, which contrasts with a previous study of the Arabidopsis pht5;1 mutant, suggesting that PHT5;1-VPTs play different roles in cellular Pi homoeostasis in seedlings of B. napus and A. thaliana. Disruption of BnPHT5;1b genes also caused Pi toxicity in floral organs, reduced seed yield and impacted seed traits, consistent with the proposed role of AtPHT5;1 in floral Pi homoeostasis in Arabidopsis. Taken together, our studies identified two vacuolar Pi influx transporters in B. napus and revealed the distinct and conserved roles of BnPHT5;1bs in cellular Pi homoeostasis in this plant species.