Potential roles of large mafs in cell lineages and developing pancreas
Potential roles of large mafs in cell lineages and developing pancreas
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DOI：
10.1097/01.mpa.0000220867.64787.99
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发表时间：
2006-05-01
期刊：
影响因子：
2.9
通讯作者：
Tsuchiya, Ken
中科院分区：
文献类型：
--
作者：
Tsuchiya, Mariko;Taniguchi, Shigeki;Tsuchiya, Ken
目的：Maf是一个具有典型bZip结构的转录因子家族，mafA是大maf家族的成员，是细胞系中胰岛素的强反式激活因子。本研究探讨大maf家族蛋白在猪胰腺组织和原代培养细胞中的表达谱。信使RNA的表达进行定量的实时聚合酶链反应，蛋白质表达进行了评估，通过Western blotting.Results：胰岛形成不清楚，在新生儿胰腺组织在成人胰腺组织。MafA和c-maf阳性细胞在胰腺组织中更广泛地定位，较少mafB阳性细胞簇分散在整个胰腺组织中。相比之下，胰岛形成更清晰，mafA和c-maf的阳性染色往往在成人胰腺组织的胰岛中更突出。mRNA和蛋白表达与免疫组化结果一致。MafA，mafB，和c-maf共表达胰岛素阳性细胞，和c-maf共表达胰高血糖素阳性细胞在成年猪胰腺的基础上的结果的双重staining study.Conclusions：大mafs被确定在正常猪和人类胰腺，和表达水平和定位的大mafs在新生儿和成人胰腺组织不同。Mats在胰腺细胞分化过程中可能在建立内分泌功能方面发挥重要作用。
Objectives: Maf is a family of transcription factor proteins characterized by a typical bZip structure, and mafA, a member of the large-maf family, is a strong transactivator of insulin in cell lines. The present study investigated the expression profiles of the large-maf family proteins in porcine pancreatic tissue and in primary culture cells.Methods: Immunohistochemical staining was performed to localize each maf protein. Messenger RNA expression was quantitated by real-time polymerase chain reaction, and protein expression was assessed by Western blotting.Results: Islet formation was not as clear in newborn pancreatic tissue as in adult pancreatic tissue. MafA- and c-maf-positive cells were more diffusely localized in pancreatic tissue with fewer mafB-positive cell clusters scattered throughout. By contrast, islet formation was clearer, and positive staining for mafA and c-maf tended to be more prominent in the islets of adult pancreatic tissue. Messenger RNA and protein expressions were consistent with the immunohistochemical findings. MafA, mafB, and c-maf coexpressed with insulin-positive cells, and c-maf coexpressed with glucagon-positive cells in adult porcine pancreas based on the results of a double-staining study.Conclusions: Large mafs were identified in normal porcine and human pancreas, and the expression levels and localizations of the large mafs in newborn and adult pancreatic tissues differed. Mats may play important roles in establishing endocrine function during pancreatic cell differentiation.