Similar morphokinetic patterns in embryos derived from obese and normoweight infertile women: a time-lapse study
Similar morphokinetic patterns in embryos derived from obese and normoweight infertile women: a time-lapse study
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DOI：
10.1093/humrep/des438
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发表时间：
2013-03-01
影响因子：
6.1
通讯作者：
Meseguer, M.
中科院分区：
文献类型：
--
作者：
Bellver, J.;Mifsud, A.;Meseguer, M.
女性肥胖是否影响延时分析评估的胚胎质量动态参数？通过图像采集和延时分析确定，女性肥胖不影响动态胚胎质量。女性肥胖损害自然生殖和辅助生殖，但对于配子、胚胎或子宫内膜容受性的具体贡献尚未达成一致。在本初步研究中，首次采用延时分析方法对胚胎质量的动态参数进行了评价。根据不孕和/或肥胖的存在，对三组患者的胚胎进行为期两年的队列回顾性研究。参与者在大学附属的私人诊所进行ICSI。我们使用一个内置摄像头的体外受精培养箱，在规定的时间点自动获取图像，监测了89名患者的个体胚胎：71个胚胎来自13名肥胖不孕妇女，242个胚胎来自45名正常体重不孕妇女，111个胚胎来自31名正常体重可育卵母细胞供体。记录细胞分裂的时间模式（细胞分裂的时间）和其他形态学特征（随时间变化的细胞大小和成核）。来自肥胖和体重正常的不孕妇女的胚胎显示出类似的卵裂模式，但发生的速度比来自有生育能力的捐赠者的胚胎慢。t2（卵裂至双卵裂球胚胎时间）、t3 （P 0.014）、t4 （P 0.003）、t5 （P 0.040）的差异均有统计学意义。这些是来自有限样本量的回顾性分析的初步数据。在对更大样本使用延时分析进行进一步研究之前，不建议使用。
Does female obesity affect the dynamic parameters of embryo quality assessed by time-lapse analysis?Female obesity does not affect the dynamic embryo quality as determined by image acquisition and time-lapse analysis.Female obesity impairs natural and assisted reproduction but there is no agreement on the specific contribution of gametes, embryos or endometrial receptivity. In this preliminary study the dynamic parameters of embryo quality are assessed for the first time by time-lapse analysis.Two-year cohort retrospective study comparing embryos from three groups of patients according to the presence of infertility and/or obesity.Participants attended a University-affiliated private clinic where ICSI was performed. Using an IVF incubator with a built-in camera designed to automatically acquire images at defined time points, we monitored individual embryos from 89 patients: 71 embryos from 13 obese infertile women, 242 embryos from 45 normoweight infertile women and 111 embryos from 31 normoweight fertile oocyte donors. The chronological pattern of cell divisions (timings of cell cleavages) and other morphologic features (time-dependent cell size and nucleation) was recorded.Embryos from obese and normoweight infertile women showed similar cleavage patterns, but occurring more slowly, to those from fertile donors. These differences were statistically significant for t2 (time of cleavage to two-blastomere embryo) (P 0.016), t3 (P 0.014), t4 (P 0.003) and t5 (P 0.040).These are preliminary data from a retrospective analysis with a limited sample size.Not recommended until further studies using time-lapse analysis of a larger sample have been performed.None.