RET TRANSFORMING GENE ENCODES A FUSION PROTEIN HOMOLOGOUS TO TYROSINE KINASES
RET TRANSFORMING GENE ENCODES A FUSION PROTEIN HOMOLOGOUS TO TYROSINE KINASES
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DOI：
10.1128/mcb.7.4.1378
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发表时间：
1987-04-01
影响因子：
5.3
通讯作者：
COOPER, GM
中科院分区：
文献类型：
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作者：
TAKAHASHI, M;COOPER, GM
ret转化基因通过两个未连接的人DNA片段之间的重组被激活，最有可能是在NIH 3 T3细胞转染期间。为了进一步确定该转化基因，我们分离并测序了ret cDNA克隆。核苷酸序列表明，活性ret转化基因编码具有羧基末端结构域的融合蛋白，该结构域与酪氨酸激酶基因家族的成员具有40 - 50%的同源性。该酪氨酸激酶结构域之前是跨膜结构域特征性的疏水序列。因此，ret酪氨酸激酶的转录可以代表在有限范围的人细胞中表达的细胞表面受体。
The ret transforming gene was activated by recombination between two unlinked segments of human DNA, most likely during transfection of NIH 3T3 cells. To further define this transforming gene, we isolated and sequenced ret cDNA clones. The nucleotide sequence indicates that the active ret transforming gene encodes a fusion protein with a carboxy-terminal domain which is 40 to 50% homologous to members of the tyrosine kinase gene family. This tyrosine kinase domain is preceded by a hydrophobic sequence characteristic of a transmembrane domain. Transcription of the ret tyrosine kinase may thus represent a cell surface receptor which is expressed in a restricted range of human cells.