Mouse models of hematological diseases using genome editing technology
Mouse models of hematological diseases using genome editing technology
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利用基因组编辑技术建立血液疾病小鼠模型
DOI：
10.11406/rinketsu.63.1551
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
稲葉 俊哉
中科院分区：
文献类型：
--
作者：
長町 安希子;稲葉 俊哉
基因编辑技术的影响已迅速扩展到开发工程。使用这种技术，小鼠的基因靶向可以在2-3个月内进行，这比使用基于胚胎干细胞的常规方法所需的时间短得多，后者需要近两年。此外，基因组编辑技术省略了几个技巧性的费力步骤。本文综述了基因打靶技术在血液学领域的突出优点。此外，作者的经验进行了审查，以确定和表征基因参与的丢失的7号染色体长臂的髓系恶性肿瘤和突出的意义，建立人类疾病的小鼠模型。
The impact of gene-editing technology has rapidly expanded into developmental engineering. Using this technology, gene targeting in mice can be performed within 2-3 months, which is a much shorter timespan than that required while using embryonic stem cell-based conventional methods, which require nearly two years. In addition, genome-editing technology omits several skillful laborious steps. This review describes the prominent merits of gene targeting using this recently established and still ongoing technology in the field of hematology. In addition, the experience of the authors is reviewed to identify and characterize genes involved in the loss of the long arm of chromosome 7 in myeloid malignancies and highlight the significance of establishing the mouse model of human diseases.