Phenotypic and genetic variation in the susceptibility of Haemophilus influenzae type b to antibodies to somatic antigens.
Phenotypic and genetic variation in the susceptibility of Haemophilus influenzae type b to antibodies to somatic antigens.
复制标题
b 型流感嗜血杆菌对体细胞抗原抗体易感性的表型和遗传变异。
DOI：
10.1172/jci109741
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
D. Smith
中科院分区：
文献类型：
--
作者：
P. Anderson;A. Flesher;S. Shaw;A. Harding;D. Smith
B型流感嗜血杆菌(H.I.b)的表型和遗传变异导致对杀菌抗体的不同敏感性已被研究。以前的研究表明，在感染大鼠体内或在大鼠血清透析液中生长后，H.I.b株EAG对抗体抗体的杀菌活性具有更强的抵抗力。我们现在报道，当EAG菌株与人血清透析液孵育时，也发生了类似的表型转变，增加的抵抗力是针对脂多糖决定簇的抗体，而不是一般的体细胞抗原，并且大多数H.I.b菌株都经历了这种转变。为了评估暴露的抗原的遗传差异，用交叉吸附的抗血清分析了从脑脊液中分离的13株H.I.b。通过6种抗原的不同表达，发现了7种不同的模式。从罕见(1：13株)到常见(10：13株)不等。至少有四个是体细胞决定簇而不是包膜决定簇；最常见的(抗原1)包含在脂多糖中。使用来自两个有记录的两个感染H.I.b的儿童的两对分离株来探索基因变异的流行病学相关性。在这两种情况下，分离株的体细胞抗原表达不同。来自一名患者的菌株在抗原1的表达上存在差异，在六种分类抗原的亚型上无法区分，但在使用患者血清鉴定的另一种抗原的表达上有所不同。
Haemophilus influenzae type b (H.i.b) has been investigated with respect to phenotypic and genetic variations resulting in differential susceptibility to bactericidal antibody. Previous studies had shown that after growth in infected rats or in dialysate of rat serum, H.i.b strain Eag became more resistant to the bactericidal activity of antisomatic antibody. We now report that a similar phenotypic shift occurs when strain Eag is incubated with dialysate of human serum, that the increased resistance is to antibodies against determinants in the lipopolysaccharide not for the somatic antigens generally, and that most strains of H.i.b undergo the shift. To assess genetic differences in exposed antigens, a panel of 13 H.i.b isolates from cerebrospinal fluid were analyzed with cross-adsorbed antisera. Seven different patterns were found that could be accounted for through the variable expression of six antigens. These ranged from infrequent (found on 1:13 strains) to common (10:13 strains). At least four were somatic rather than capsular determinants; the most common (antigen 1) was contained in lipopolysaccharide. The epidemiologic relevance of the genetic variations was explored using pairs of isolates from two children who had had two documented infections with H.i.b. In both cases the isolates varied in somatic antigen expression. The strains from one patient differed in the expression of antigen 1. The isolates from the other were indistinguishable in sub-typing for the six classified antigens, but differed in the expression of an additional antigen identified by use of the patient's serum.