Insulin Resistance, Microbiota, and Fat Distribution Changes by a New Model of Vertical Sleeve Gastrectomy in Obese Rats
Insulin Resistance, Microbiota, and Fat Distribution Changes by a New Model of Vertical Sleeve Gastrectomy in Obese Rats
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DOI：
10.2337/db16-0039
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发表时间：
2016-10-01
期刊：
影响因子：
7.7
通讯作者：
Mingrone, Geltrude
中科院分区：
文献类型：
--
作者：
Basso, Nicola;Soricelli, Emanuele;Mingrone, Geltrude
新陈代谢手术改善胰岛素抵抗和2型糖尿病，可能是因为体重减轻。我们在大鼠身上进行了一种新的袖状胃切除术，切除了类似于腺体部分的80%，保留了几乎完整的前胃(腺体胃切除术[GG])，并将随后的代谢重建与假手术进行了比较。GG对体重没有影响，至少在10周后；可能通过增加Akt、糖原合成酶激酶3和AMPK磷酸化来改善肝脏和外周胰岛素的敏感性；减少异位脂肪沉积和肝糖原过度积聚。体脂组织重新分布，腹内脂肪减少。我们发现循环中的Ghrelin水平降低，GLP-1血浆浓度增加，肠道微生物组多样性重塑，其特征是瘤胃球菌的相对丰度较低，乳杆菌和Collinsella的相对丰度较高。这些数据表明，至少在老鼠身上，腺胃在改善胰岛素抵抗方面发挥着核心作用，即使肥胖症持续存在。GG为代谢健康的肥胖表型提供了一个新的模型。
Metabolic surgery improves insulin resistance and type 2 diabetes possibly because of weight loss. We performed a novel sleeve gastrectomy in rats that resects similar to 80% of the glandular portion, leaving the forestomach almost intact (glandular gastrectomy [GG]) and compared subsequent metabolic remodeling with a sham operation. GG did not affect body weight, at least after 10 weeks; improved hepatic and peripheral insulin sensitivity likely through increased Akt, glycogen synthase kinase 3, and AMPK phosphorylation; and reduced ectopic fat deposition and hepatic glycogen overaccumulation. Body adipose tissue was redistributed, with reduction of intraabdominal fat. We found a reduction of circulating ghrelin levels, increased GLP-1 plasma concentration, and remodeling of gut microbiome diversity characterized by a lower relative abundance of Ruminococcus and a higher relative abundance of Lactobacillus and Collinsella. These data suggest that at least in rat, the glandular stomach plays a central role in the improvement of insulin resistance, even if obesity persists. GG provides a new model of the metabolically healthy obese phenotype.