Adaptation of FUN-1 and Calcofluor white stains to assess the ability of viable and nonviable yeast to adhere to and be internalized by cultured mammalian cells
Adaptation of FUN-1 and Calcofluor white stains to assess the ability of viable and nonviable yeast to adhere to and be internalized by cultured mammalian cells
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DOI：
10.1016/j.mimet.2004.07.001
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发表时间：
2004-11-01
影响因子：
2.2
通讯作者：
Wells, CL
中科院分区：
文献类型：
--
作者：
Henry-Stanley, MJ;Garni, RM;Wells, CL
FUN-1 和 Calcoflor 白色染色剂可协同使用，以评估活酵母和非活酵母在体外粘附宿主哺乳动物细胞并被宿主哺乳动物细胞内化的能力。该方法仅用Calcoflor对酵母细胞外进行染色，死酵母细胞具有弥散的细胞质黄绿色荧光，而活酵母细胞具有细胞质橙红色或黄橙色荧光空泡内结构。 (C) 2004 Elsevier B.V. 保留所有权利。
The FUN-1 and Calcofluor white stains can be used in concert to assess the ability of viable and nonviable yeast to adhere to, and be internalized by, host mammalian cells in vitro. With this method, only extracellular yeast stain with Calcofluor, dead yeast cells have diffuse cytoplasmic yellow-green fluorescence, and live yeast have cytoplasmic orange-red or yellow-orange fluorescent intravacuolar structures. (C) 2004 Elsevier B.V. All rights reserved.