Aquaporin-2 plays an important role in water transportation through the bladder wall in rats
Aquaporin-2 plays an important role in water transportation through the bladder wall in rats
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DOI：
10.1002/nau.23715
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发表时间：
2018-11-01
影响因子：
2
通讯作者：
Fujimoto, Kiyohide
中科院分区：
文献类型：
--
作者：
Morizawa, Yosuke;Torimoto, Kazumasa;Fujimoto, Kiyohide
目的研究膀胱壁对水的渗透作用，以水通道蛋白为研究重点，选用体重300g的雌性SD大鼠，研究膀胱壁在生理盐水渗透中的作用。分别于给药后3h测定去氨加压素水合物组和对照组膀胱内液体量和钠浓度的变化。切除膀胱，用qRT-PCR检测水通道蛋白-1、2、3基因的表达。结果157.80~3h膀胱内液体量和钠浓度均显著下降(1.000.00vs0.83+/-0.08mL，0.83+/-1.30vs 146.8+/-1.92mEq/mL，P
AimMethodsWe investigated the role of the bladder wall in permeating water, focusing on aquaporins.Female Sprague-Dawley rats weighing 300g were used to investigate the role of the bladder wall in saline permeation. Changes in intravesical fluid volume and sodium concentration were measured in the desmopressin acetate hydrate-loaded and control groups 3h after administration. Bladders were resected to measure aquaporin-1, 2, and 3 gene expression using qRT-PCR. Additionally, the change of aquaporin-2 expression was measured using Western blotting and immunohistochemistry in intravesical aquaporin-2 siRNA-treated and control groups.ResultsConclusionsAlthough the intravesical fluid volume and sodium concentration significantly decreased from 0 to 3h (1.000.00 vs 0.83 +/- 0.08mL, 157.80 +/- 1.30 vs 146.8 +/- 1.92mEq/mL, P