Regeneration of irradiated salivary glands with stem cell marker expressing cells
Regeneration of irradiated salivary glands with stem cell marker expressing cells
复制标题
DOI：
10.1016/j.radonc.2011.05.085
复制
发表时间：
2011-06-01
影响因子：
5.7
通讯作者：
Coppes, Robert P.
中科院分区：
文献类型：
--
作者：
Nanduri, Lalitha S. Y.;Maimets, Martti;Coppes, Robert P.
背景：干细胞治疗可能是一种潜在的减少放射性低唾液分泌和改善患者生活质量的方法。然而，唾液腺干细胞的鉴定和纯化尚未完成。本研究旨在更好地表征干/祖细胞群体与再生潜力居住在小鼠salivary gland.Methods：小鼠下颌下腺组织，分离的细胞和培养的3天龄salispheres进行了测试，他们的干细胞标志物c-Kit，CD 133，CD 49 f，和CD 24的表达，使用免疫组织化学组织和流式细胞术的细胞。小鼠局部照射与单剂量的15戈伊和移植与细胞表达定义markets.Results：细胞表达已知的干细胞标记物定位在小鼠唾液腺的较大导管。分离的细胞和来自第3天盐球的细胞也表达这些标志物：c-Kit（0.058%对0.65%）、CD 133（6%对5%）、CD 49 f（78%对51%）和CD 24（分别为60%对60%）。腺内移植这些细胞到照射小鼠唾液腺导致干细胞标志物特异性的恢复唾液腺functions.Conclusions：不同的干细胞相关的标志物表达在小鼠唾液腺细胞，移植后能够再生照射损伤的唾液腺。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。放射治疗和肿瘤学99（2011）367-372
Background: Stem cell therapy could be a potential way for reducing radiation-induced hyposalivation and improving the patient's quality of life. However, the identification and purification of salivary gland stem cells have not been accomplished. This study aims to better characterize the stem/progenitor cell population with regenerative potential residing in the mouse salivary gland.Methods: Mouse submandibular gland tissue, isolated cells and cultured 3 day old salispheres were tested for their expression of stem cell markers c-Kit, CD133, CD49f, and CD24 using immunohistochemistry for tissue and flow cytometry for cells. Mice were locally irradiated with a single dose of 15 Gy and transplanted with cells expressing defined markers.Results: Cells expressing known stem cell markers are localized in the larger ducts of the mouse salivary gland. Isolated cells and cells from day 3 salispheres also express these markers: c-Kit (0.058% vs. 0.65%), CD133 (6% vs. 5%), CD49f (78% vs. 51%), and CD24 (60% vs. 60%, respectively). Intraglandular transplantation of these cells into irradiated salivary glands of mice resulted in stem cell marker-specific recovery of salivary gland function.Conclusions: Different stem cell-associated markers are expressed in mouse salivary gland cells, which upon transplantation are able to regenerate the irradiation damaged salivary gland. (C) 2011 Elsevier Ireland Ltd. All rights reserved. Radiotherapy and Oncology 99 (2011) 367-372