FUNCTIONAL AND STRUCTURAL CHARACTERIZATION OF P40, A MOUSE GLYCOPROTEIN WITH T-CELL GROWTH-FACTOR ACTIVITY
FUNCTIONAL AND STRUCTURAL CHARACTERIZATION OF P40, A MOUSE GLYCOPROTEIN WITH T-CELL GROWTH-FACTOR ACTIVITY
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DOI：
10.1073/pnas.85.18.6934
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发表时间：
1988-09-01
影响因子：
11.1
通讯作者：
VANSNICK, J
中科院分区：
文献类型：
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作者：
UYTTENHOVE, C;SIMPSON, RJ;VANSNICK, J
从小鼠辅助性T细胞克隆经刀豆蛋白A刺激后获得的自体上清液中培养获得抗原非依赖性细胞系，纯化了一种支持这些细胞系生长的因子，命名为P40，并鉴定为一种基本的32-39 kDa的单链糖蛋白，其功能与先前已知的T细胞生长因子不同，在结构上显然与任何已知的蛋白无关。在许多细胞系中，只有辅助性T细胞对P40有反应，这种反应不是由白介素2或白介素4介导的。
Antigen-independent cell lines were derived from mouse helper T-cell clones by culture in autologous supernatant obtained after stimulation with concanavalin A.A factor, termed P40, supporting the growth of these lines was purified and characterized as a basic 32- to 39-kDa single-chain glycoprotein functionally distinct from previously identified T-cell growth factors and apparently unrelated structurally to any known protein. Of a number of cell lines, only helper T cells responded to P40, and this response was not mediated by either interleukin 2 or interleukin 4.