Mapping of hybrid incompatibility loci in Nasonia.
Mapping of hybrid incompatibility loci in Nasonia.
复制标题
Nasonia 杂种不亲和基因座的定位。
DOI：
10.1093/genetics/153.4.1731
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发表时间：
1999
期刊：
影响因子：
3.3
通讯作者：
Werren,JH
中科院分区：
文献类型：
--
作者：
Gadau,J;PageJr,RE;Werren,JH
根据理论，F2杂种的崩溃(致死或不育)是由于不同物种之间相互作用的基因之间的不亲和性(即，共适应基因复合体的破坏)。在单倍体物种中检测这种不亲和性特别简单，因为处女F1杂交雌性将产生单倍体重组F2雄性。这一特征允许对整个基因组进行隐性遗传不亲和性的筛选。以丽纹夜蛾为材料，与其近缘种吉拉乌尔蒂夜蛾杂交。首先，利用随机扩增多态性DNA标记构建了一张连锁图谱。随机扩增多态性DNA标记显示总体偏向于玻璃虫等位基因，这一模式没有被基本的双交互作用Dobzhansky-Muller模型预测到。可见标记和连锁标记的回收模式是一致的。如果两个基因座之间的特定遗传交互作用导致了杂交致死，那么这些基因型在成年F2男性中应该是代表不足或不存在的。通过对不同染色体上的标记进行配对比较，发现了四组显著的不亲和性。对观察到的模式的可能解释是母性效应-合子基因不相容或不相容基因的聚集。由于世代时间短、单倍体的优势和标记的可获得性，NASONIA有望成为研究杂种不育遗传学的高产系统。
According to theory, F2hybrid breakdown (lethality or sterility) is due to incompatibilities between interacting genes of the different species (i.e., the breaking up of coadapted gene complexes). Detection of such incompatibilities is particularly straightforward in haplodiploid species, because virgin F1hybrid females will produce haploid recombinant F2males. This feature allows for screening of the complete genome for recessive genetic incompatibilities. Crosses were performed between Nasonia vitripennis (v) and its sibling species N. giraulti (g). First, a linkage map was produced using RAPD markers. RAPD markers showed an overall bias toward vitripennis alleles, a pattern not predicted by the basic two-interactor Dobzhansky-Muller model. Recovery patterns of visible markers were consistent with those of linked RAPD markers. If particular genetic interactions between two loci are causing hybrid lethality, then those genotypes should be underrepresented or absent among adult F2males. Four sets of significant incompatibilities were detected by performing pairwise comparisons of markers on different chromosomes. Likely explanations for the observed patterns are maternal effect-zygotic gene incompatibilities or clustering of incompatibility loci. Due to the short generation time, advantages of haplodiploidy, and availability of markers, Nasonia promises to be a productive system for investigating the genetics of hybrid inviability.