Three-dimensional reconstruction of collagen-proteoglycan interactions in the mouse corneal stroma by electron tomography
Three-dimensional reconstruction of collagen-proteoglycan interactions in the mouse corneal stroma by electron tomography
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DOI：
10.1016/j.jsb.2010.01.019
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发表时间：
2010-05-01
影响因子：
3
通讯作者：
Knupp, Carlo
中科院分区：
文献类型：
--
作者：
Parfitt, Geraint J.;Pinali, Christian;Knupp, Carlo
角膜透明是视觉系统的基础，并且与角膜维持的有序胶原纤维结构直接相关。蛋白聚糖，通过其蛋白核心和高阴离子糖胺聚糖侧链，被认为调节角膜基质中的胶原组织。为了了解角膜中蛋白聚糖和胶原原纤维之间的相互关系，我们用cuprolinic blue处理成年小鼠角膜，获得角膜前、中、后基质的三维重建。重建显示整个角膜的胶原原纤维直径规则，每个区域内纤维间距均匀。通过纵向和横向的重建，建立了清晰的蛋白多糖组织图，但没有观察到明显的规则模式或对称的蛋白多糖取向。大的，电子密集的蛋白聚糖（可能是硫酸软骨素/硫酸皮肤蛋白聚糖）可以连接两个或三个相邻的胶原原纤维，而另一个较小的蛋白聚糖亚群（硫酸角蛋白品种）只连接相邻的原纤维。重建表明蛋白聚糖和胶原蛋白之间存在复杂的相互作用，这使得角膜中胶原纤维结构的动态控制成为可能。(C) 2010爱思唯尔公司版权所有。
Corneal transparency is fundamental to the visual system, and is directly related to the ordered collagen fibril architecture that the cornea maintains. Proteoglycans, through their protein core and highly anionic glycosaminoglycan side chains, are thought to regulate the collagen organisation in the corneal stroma. To understand the inter-relationships between proteoglycans and collagen fibrils in the cornea, adult mouse corneas were treated with cuprolinic blue and three-dimensional reconstructions of the anterior, mid and posterior corneal stroma were obtained. The reconstructions show regular diameters of collagen fibrils throughout the cornea and uniform interfibrillar spacing within each region. Both longitudinal and transverse reconstructions were obtained to establish a clear picture of proteoglycan organisation, yet no distinct regular pattern or symmetry of proteoglycan orientation was observed. Large, electron-dense proteoglycans (possibly chondroitin sulphate/dermatan sulphate proteoglycans) interconnecting two or often three adjacent collagen fibrils are seen, whilst another sub-population of smaller proteoglycans (of the keratan sulphate variety) interconnect only neighbouring fibrils. The reconstructions suggest a complex interaction between proteoglycans and collagen, which allows for the dynamic control of collagen fibril architecture in the cornea. (C) 2010 Elsevier Inc. All rights reserved.