Structures of the human and Drosophila 80S ribosome
Structures of the human and Drosophila 80S ribosome
复制标题
DOI：
10.1038/nature12104
复制
发表时间：
2013-05-02
期刊：
影响因子：
64.8
通讯作者：
Beckmann, Roland
中科院分区：
文献类型：
--
作者：
Anger, Andreas M.;Armache, Jean-Paul;Beckmann, Roland
所有细胞中的蛋白质合成都是由称为核糖体的大分子机器进行的。虽然原核生物、酵母和原生生物核糖体的结构已经确定，但后生动物80S核糖体更复杂的分子结构到目前为止仍然难以捉摸。在这里，我们展示了果蝇和智人80S核糖体与翻译因子eEF2，E位点转移RNA和Stm1样蛋白的复合体结构，基于高分辨率冷冻电子显微镜密度图。这些结构不仅说明了后生动物特有的核糖体RNA与核糖体蛋白的共同进化，还揭示了后生动物核糖体中存在两个额外的结构层，一个有序的内层被一个柔性的RNA外层覆盖。人类和果蝇的核糖体结构将为更详细的结构、生化和遗传学实验提供基础。
Protein synthesis in all cells is carried out by macromolecular machines called ribosomes. Although the structures of prokaryotic, yeast and protist ribosomes have been determined, the more complex molecular architecture of metazoan 80S ribosomes has so far remained elusive. Here we present structures of Drosophila melanogaster and Homo sapiens 80S ribosomes in complex with the translation factor eEF2, E-site transfer RNA and Stm1-like proteins, based on high-resolution cryo-electron-microscopy density maps. These structures not only illustrate the co-evolution of metazoan-specific ribosomal RNA with ribosomal proteins but also reveal the presence of two additional structural layers in metazoan ribosomes, a well-ordered inner layer covered by a flexible RNA outer layer. The human and Drosophila ribosome structures will provide the basis for more detailed structural, biochemical and genetic experiments.