Evaluation of the cytotoxic effects of hyperthermia and 5-fluorouracil-loaded magnetic nanoparticles on human colon cancer cell line HT-29
Evaluation of the cytotoxic effects of hyperthermia and 5-fluorouracil-loaded magnetic nanoparticles on human colon cancer cell line HT-29
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DOI：
10.1080/02656736.2016.1243260
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发表时间：
2017-05-01
影响因子：
3.1
通讯作者：
Esmaelbeygi, Elaheh
中科院分区：
文献类型：
--
作者：
Eynali, Samira;Khoei, Samideh;Esmaelbeygi, Elaheh
本研究的目的是评价热与聚乳酸-羟基乙酸（PLGA）纳米颗粒作为5-氟尿嘧啶载体（带/不带氧化铁核）对人结肠癌HT-29细胞在球体模型中的生存能力和增殖能力的联合影响。用不同浓度的5-FU或将5-FU装载到两种纳米颗粒中处理HT-29球形细胞74h。然后在43℃下进行热疗60分钟。最后，分别用台锥蓝染色排除试验和菌落形成试验评估上述处理对细胞活力和增殖能力的影响。我们的研究结果表明，与对照组相比，热疗联合5-FU或PLGA纳米颗粒作为5-FU载体，显著增强了细胞毒作用。考虑到纳米颗粒可以增加癌细胞细胞内药物浓度，两种纳米颗粒均加载5-FU治疗后的细胞毒性作用程度明显高于游离5-FU。此外，与不含氧化铁核的纳米颗粒相比，热疗期间纳米颗粒中氧化铁核的存在增强了热疗的细胞毒性作用。基于本研究，高温联合负载5- fu的PLGA纳米颗粒氧化铁核显著降低HT-29细胞的增殖能力；因此，它可能被认为是结肠癌治疗的一个新方向。
The purpose of this study was to evaluate the combined effects of heat and polylactic-co-glycolic acid (PLGA) nanoparticles, as 5-fluorouracil carriers with/without iron oxide core, on the viability and proliferation capacity of human colon cancer cell line HT-29 in the spheroid model. HT-29 spheroid cells were treated with different concentrations of 5-FU or 5-FU-loaded into both nanoparticles for 74h. Hyperthermia was then performed at 43 degrees C for 60min. Finally, the effects of the mentioned treatments on cell viability and proliferation capacity were evaluated using the trypan blue dye exclusion test and colony formation assay, respectively. Our results showed that hyperthermia, in combination with 5-FU or PLGA nanoparticles as 5-FU carriers, significantly enhanced the cytotoxic effects as compared to the control group. Considering that nanoparticles could increase the intracellular concentration of drugs in cancer cells, the extent of cytotoxic effects following treatment with 5-FU-loaded into both nanoparticles was significantly higher than that with free 5-FU. In addition, the presence of iron oxide cores in nanoparticles during hyperthermia enhanced the cytotoxic effects of hyperthermia compared with nanoparticles without iron oxide core. Based on this study, hyperthermia in combination with 5-FU-loaded PLGA nanoparticles with iron oxide core drastically reduced the proliferation capacity of HT-29 cells; therefore, it may be considered as a new direction in the treatment of colon cancer.