Binding of 21 thiol reagents to human hemoglobin in solution and in intact cells.
Binding of 21 thiol reagents to human hemoglobin in solution and in intact cells.
复制标题
21 种硫醇试剂与溶液和完整细胞中的人血红蛋白结合。
DOI：
10.1111/j.1432-1033.1982.tb06561.x
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
J. Rosa
中科院分区：
文献类型：
--
作者：
M. Garel;Y. Beuzard;J. Thillet;C. Domenget;J. Martin;F. Galactéros;J. Rosa
The reactivity of the cysteine-beta 93 residue of human hemoglobin was investigated in order to define the optimal structure of potential antisickling agents. The properties of 21 thiol reagents were compared with regard to (a) their binding rate to hemoglobin in solution and within intact cells; (b) the modification of the oxygen dissociation curve of intact cells and (c) the effect on methemoglobin formation in solution or within intact cells. The results show the very different behaviors of these reagents.
