T lineage differentiation from human embryonic stem cells
T lineage differentiation from human embryonic stem cells
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DOI：
10.1073/pnas.0604244103
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发表时间：
2006-08-01
影响因子：
11.1
通讯作者：
Zack, Jerome A.
中科院分区：
文献类型：
--
作者：
Galic, Zoran;Kitchen, Scott G.;Zack, Jerome A.
利用基因操纵的人类胚胎干细胞（hESC）分化成适当的谱系的能力可以彻底改变医疗实践。这些细胞在理论上具有发展成所有成熟细胞类型的潜力；然而，发展成所有造血谱系的实际能力尚未得到证实。通过小鼠骨髓基质细胞体外连续共培养，以及移植到免疫缺陷小鼠胸腺组织中，我们证明hESC可以通过T淋巴系分化。在整个分化过程中，稳定的转基因表达保持在高水平，这表明基因操纵的hESC具有治疗多种T细胞疾病的潜力。
Harnessing the ability of genetically manipulated human embryonic stem cells (hESC) to differentiate into appropriate lineages could revolutionize medical practice. These cells have the theoretical potential to develop into all mature cell types; however, the actual ability to develop into all hematopoietic lineages has not been demonstrated. Using sequential in vitro coculture on murine bone marrow stromal cells, and engraftment into human thymic tissues in immunodeficient mice, we demonstrate that hESC can differentiate through the T lymphoid lineage. Stable transgene expression was maintained at high levels throughout differentiation, suggesting that genetically manipulated hESC hold potential to treat several T cell disorders.