HUMAN TAF(II)31 PROTEIN IS A TRANSCRIPTIONAL COACTIVATOR OF THE P53 PROTEIN
HUMAN TAF(II)31 PROTEIN IS A TRANSCRIPTIONAL COACTIVATOR OF THE P53 PROTEIN
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DOI：
10.1073/pnas.92.11.5154
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发表时间：
1995-05-23
影响因子：
11.1
通讯作者：
LEVINE, AJ
中科院分区：
文献类型：
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作者：
LU, H;LEVINE, AJ
p53蛋白通过与特异性DNA反应元件结合并与RNA聚合酶II的转录装置相互作用来激活靶基因的转录。p53的氨基末端结构域与TFIID基础转录复合物的组分相互作用。人TATA结合蛋白相关因子TAF（II）31是TFIID的一个组分，已被鉴定为p53介导的转录激活所需的关键蛋白。TAF（II）31和p53蛋白通过p53氨基末端结构域中的氨基酸残基相互结合，这些氨基酸残基对于转录是必需的。针对TAF（II）31蛋白的抗体在体外抑制p53激活的但不抑制基础转录。这些结果表明，TAF（II）31是p53蛋白的共活化剂。
The p53 protein activates transcription of a target gene by binding to a specific DNA response element and interacting with the transcriptional apparatus of RNA polymerase II. The amino-terminal domain of p53 interacts with a component of the TFIID basal transcription complex. The human TATA-binding-protein-associated factor TAF(II)31, a component of TFIID, has been identified as a critical protein required for p53-mediated transcriptional activation. TAF(II)31 and p53 proteins bind to each other via amino acid residues in the amino-terminal domain of p53 that are essential for transcription. Antibodies directed against TAF(II)31 protein inhibit p53-activated but not basal transcription in vitro. These results demonstrate that TAF(II)31 is a coactivator for the p53 protein.