See-saw rocking: an in vitro model for mechanotransduction research
See-saw rocking: an in vitro model for mechanotransduction research
复制标题
跷跷板摇摆：力传导研究的体外模型
DOI：
10.1098/rsif.2014.0330
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发表时间：
2014-08
影响因子：
3.9
通讯作者：
Thompson, M. S.
中科院分区：
文献类型：
--
作者：
Chen, D.;Ventikos, Y.;Bomphrey, R. J.;Thompson, M. S.
体外机械力传递研究揭示了细胞对机械力响应的基础科学，对于组织工程的进展及其临床应用至关重要。许多不同的研究已经描述了大量的细胞反应;然而，由于所施加的应力的精确性质和大小很少被报道并且很少被验证，因此实验通常不具有可比性，从而限制了研究进展。本文提供了一种广泛使用的流体刺激装置，跷跷板摇杆，作为循环流体剪切应力机械转导的体外模型的物理和生物学验证。这允许在方便的六孔板格式中精确表征的刺激和细胞单层反应之间的联系。使用计算流体动力学对一口井的模型进行了离散化和广泛分析，以在0.5 Hz的摇摆频率下产生循环流体速度矢量的收敛、稳定和一致的预测，并考虑到自由表面。通过与使用粒子图像测速仪实验测得的流速进行比较，提供了验证。定性流动行为相匹配，定量分析表明，在代表性的位置和时间点的协议。最大剪切应力估计为0.22 Pa，靠近井边缘，时间平均剪切应力范围在0.029和0.068 Pa之间。使用该系统刺激的人腱细胞在第2天和第7天的时间点显示胶原和GAG分泌显著增加。这种体外力学转导模型为研究流体剪切应力对生物细胞的影响提供了一种通用、灵活和廉价的方法。
In vitro mechanotransduction studies, uncovering the basic science of the response of cells to mechanical forces, are essential for progress in tissue engineering and its clinical application. Many varying investigations have described a multitude of cell responses; however, as the precise nature and magnitude of the stresses applied are infrequently reported and rarely validated, the experiments are often not comparable, limiting research progress. This paper provides physical and biological validation of a widely available fluid stimulation device, a see-saw rocker, as an in vitro model for cyclic fluid shear stress mechanotransduction. This allows linkage between precisely characterized stimuli and cell monolayer response in a convenient six-well plate format. Models of one well were discretized and analysed extensively using computational fluid dynamics to generate convergent, stable and consistent predictions of the cyclic fluid velocity vectors at a rocking frequency of 0.5 Hz, accounting for the free surface. Validation was provided by comparison with flow velocities measured experimentally using particle image velocimetry. Qualitative flow behaviour was matched and quantitative analysis showed agreement at representative locations and time points. Maximum shear stress of 0.22 Pa was estimated near the well edge, and time-average shear stress ranged between 0.029 and 0.068 Pa. Human tenocytes stimulated using the system showed significant increases in collagen and GAG secretion at 2 and 7 day time points. This in vitro model for mechanotransduction provides a versatile, flexible and inexpensive method for the fluid shear stress impact on biological cells to be studied.
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影响因子：
3.5
作者：
M. Thompson;Stuart Abercrombie;C. Ott;F. Bieler;G. Duda;Y. Ventikos
通讯作者：
M. Thompson;Stuart Abercrombie;C. Ott;F. Bieler;G. Duda;Y. Ventikos
DOI：
--
发表时间：
1986-08
期刊：
Iyo denshi to seitai kogaku. Japanese journal of medical electronics and biological engineering
影响因子：
--
作者：
M. Sato
通讯作者：
M. Sato
DOI：
10.1016/j.bbrc.2011.07.072
发表时间：
2011-08-19
影响因子：
3.1
作者：
Hoey, David A.;Kelly, Daniel J.;Jacobs, Christopher R.
通讯作者：
Jacobs, Christopher R.
影响因子：
3.9
作者：
Anderson EJ;Knothe Tate ML
通讯作者：
Knothe Tate ML
DOI：
10.1115/1.3138285
发表时间：
1981-07
影响因子：
1.7
作者：
Y. Fung;R. Skalak
通讯作者：
Y. Fung;R. Skalak