A SOLVENT PARTITION METHOD FOR MICROSCALE GANGLIOSIDE PURIFICATION
A SOLVENT PARTITION METHOD FOR MICROSCALE GANGLIOSIDE PURIFICATION
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DOI：
10.1016/0003-2697(85)90419-1
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发表时间：
1985-01-01
影响因子：
2.9
通讯作者：
GILLARD, B
中科院分区：
文献类型：
--
作者：
LADISCH, S;GILLARD, B
描述了一种从人血浆、细胞或组织的总脂质提取物中纯化神经节苷脂的简单而快速的方法。该方法的新颖之处在于将干燥的总脂质提取物分配在由二异丙醚、1-丁醇和 50 mM 氯化钠水溶液（6/4/5，v/v/v [vol/vol/vol]）组成的 3 室溶剂系统中。神经节苷脂几乎定量地分配到下层水相中，而其他脂质则分配到上层有机相中，这是由这 3 种溶剂的混合物产生的。然后通过凝胶过滤将含有神经节苷脂的水相除去盐和其他低MW杂质。由此获得的总神经节苷脂的 TLC 图案清晰可见，即使通过该方法处理神经节苷脂浓度非常低的小样品（例如 1 ml 血浆样品）也是如此。这种新的神经节苷脂纯化方法特别适用于需要分析多个小样品的神经节苷脂的比较定性研究。
A simple and rapid method for the purification of gangliosides from the total lipid extract of human plasma, cells, or tissue is described. The novel component of the method is the partition of the dried total lipid extract in the 3-compartment solvent system consisting of diisopropyl ether, 1-butanol and 50 mM aqueous NaCl (6/4/5, v/v/v [vol/vol/vol]). Gangliosides partition nearly quantitatively into the lower aqueous phase, and other lipids into the upper organic phase, resulting from the mixture of these 3 solvents. The ganglioside-containing aqueous phase is then freed of salts and other low MW impurities by gel filtration. The TLC patterns of total gangliosides thus obtained are clear and distinct, even when small samples with very low ganglioside concentrations (e.g., 1-ml samples of plasma) are processed by this method. This new ganglioside purification method is especially applicable to comparative qualitative studies of gangliosides requiring the analysis of multiple small samples.