In Situ Ratiometric Fluorescence Imaging for Tracking Targeted Delivery and Release of Anticancer Drug in Living Tumor Cells.
In Situ Ratiometric Fluorescence Imaging for Tracking Targeted Delivery and Release of Anticancer Drug in Living Tumor Cells.
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DOI：
10.1021/acsabm.9b00737
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发表时间：
2019-09
影响因子：
4.7
通讯作者：
Keqin Yang;Jingjin Zhao;Liangliang Zhang;Rongjun Liu;H. Liang;Shulin Zhao
中科院分区：
文献类型：
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作者：
Keqin Yang;Jingjin Zhao;Liangliang Zhang;Rongjun Liu;H. Liang;Shulin Zhao
原位比率荧光追踪抗肿瘤药物的递送和释放仍然是癌症靶向治疗的挑战。在此，我们制备了适配体功能化的生物质量子点（BQD-Apt）作为纳米载体和探针，用于跟踪阿霉素（DOX）在活细胞中的递送和释放。BQD-Apt-DOX复合物中BQDs与DOX之间的荧光共振能量转移在释放DOX后被抑制，实现了DOX传递和释放的原位比率荧光成像。由于核酸适体良好的肿瘤靶向性和BQDs的生物相容性，BQD-Apt-DOX具有提高肿瘤治疗效率的巨大潜力。
In situ ratiometric fluorescence tracking the delivery and release of antitumor drugs remains a challenge for targeted treatment of cancer. Herein, we fabricated aptamer-functionalized biomass quantum dots (BQD-Apt) as the nanocarriers and probes for tracking the delivery and release of doxorubicin (DOX) in living cells. The fluorescence resonance energy transfer between BQDs and DOX in the formed BQD-Apt-DOX complex was inhibited after releasing DOX, achieving in situ ratiometric fluorescence imaging of delivery and release of DOX. Because of the good cancer-targeting ability of aptamer and biocompatibility of BQDs, BQD-Apt-DOX holds great potential for improving the efficiency of cancer treatment.